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PMID: 1545827 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The retinoblastoma protein physically associates with the human cdc2 kinase.

Molecular and cellular biology ·Vol. 12 ·No. 3 ·1992-03-00 ·Pages 971-80

Hu QJ, Lees JA, Buchkovich KJ, Harlow E

Abstract

The protein product (pRB) of the retinoblastoma susceptibility gene functions as a negative regulator of cell proliferation, and its activity appears to be modulated by phosphorylation. Using a new panel of anti-human pRB monoclonal antibodies, we have investigated the biochemical properties of this protein. These antibodies have allowed us to detect a pRB-associated kinase that has been identified as the cell cycle-regulating kinase p34cdc2 or a closely related enzyme. Since this associated kinase phosphorylates pRB at most of the sites used in vivo, these results suggest that this kinase is one of the major regulators of pRB. The associated kinase activity follows the pattern of phosphorylation seen for pRB in vivo. The associated kinase activity is not seen in the G1 phase but appears in the S phase, and the levels continue to increase throughout the remainder of the cell cycle.

MeSH Terms
Antibodies, Monoclonal Blotting, Western CDC2 Protein Kinase/metabolism Cell Division Cells, Cultured Electrophoresis, Gel, Two-Dimensional Humans Peptide Mapping Phosphorylation Precipitin Tests Retinoblastoma Protein/immunology,metabolism Substrate Specificity
Chemicals
Antibodies, Monoclonal Retinoblastoma Protein CDC2 Protein Kinase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hu Q J
Cold Spring Harbor Laboratory, New York 11724.
Lees J A
Buchkovich K J
Harlow E
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.3.971-980.1992
Published
1992-03-00
Pages
971-80
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369529
Subset
IM
Grants
NCI NIH HHS · CA13106 · United States
External Links
PubMed source DOI: 10.1128/mcb.12.3.971-980.1992 PMC Full Text View journal details
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