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PMID: 8035487 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The extreme carboxyl terminus of the equine herpesvirus 1 homolog of herpes simplex virus VP16 is essential for immediate-early gene activation.

Journal of virology ·Vol. 68 ·No. 8 ·1994-08-00 ·Pages 4890-7

Elliott GD

Abstract

Gene 12 of equine herpesvirus 1 (EHV-1), the homolog of herpes simplex virus (HSV) VP16 (alpha TIF, Vmw65), was cloned into a eukaryotic expression vector by PCR and used in transactivation studies of both the EHV-1 and HSV-1 IE1 promoters. Results demonstrated that the product of gene 12 is a potent transactivator of immediate-early gene expression of both viruses, which requires sequences in the upstream HSV-1 promoter for activity. Mutational analysis of the gene 12 open reading frame indicated that removal of the C-terminal 7 amino acids, which contain a short region of homology with the extreme C terminus of VP16, inactivated the protein. Within this region, only a single methionine residue appeared to be essential for activity, implying that gene 12 may have a modular array of organization similar to that of VP16. However, fusion of the gene 12 C terminus to a truncated form of VP16, which contained the complex formation domain, did not restore activity to the HSV-1 protein. These data demonstrate that the EHV-1 immediate-early transactivator may not be functionally colinear with VP16, with transactivation requiring both the C terminus and another region(s) present within the N-terminal portion.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular DNA, Viral Gene Expression Regulation, Viral Herpes Simplex Virus Protein Vmw65/chemistry,genetics Herpesvirus 1, Equid/genetics Immediate-Early Proteins/genetics Methionine/metabolism Molecular Sequence Data Promoter Regions, Genetic Sequence Homology Transcriptional Activation Vero Cells
Chemicals
DNA, Viral Herpes Simplex Virus Protein Vmw65 Immediate-Early Proteins Methionine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Elliott G D
Department of Microbiology, University of Leeds, United Kingdom.
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.68.8.4890-4897.1994
Published
1994-08-00
Pages
4890-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC236429
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1128/JVI.68.8.4890-4897.1994 PMC Full Text View journal details
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