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PMID: 2854058 Published · ppublish English Journal Article

Direct combinatorial interaction between a herpes simplex virus regulatory protein and a cellular octamer-binding factor mediates specific induction of virus immediate-early gene expression.

The EMBO journal ·Vol. 7 ·No. 13 ·1988-12-20 ·Pages 4231-8

O'Hare P, Goding CR, Haigh A

Abstract

We provide evidence for a novel mechanism of transcriptional regulation in which the immediate-early (IE) transactivating protein of herpes simplex virus, Vmw65, is assembled into a specific DNA-binding complex together with a cellular octamer-binding factor (TRF). The assembly of Vmw65/TRF complex requires not only the core TRF recognition site, but also flanking sequences which are dispensable for TRF binding alone. We show from functional analyses that TRF binding by a motif is required but not sufficient to confer induction on a heterologous promoter, and it is the ability of the motif to allow TRF/Vmw65 complex assembly which correlates with functional activity. Thus, for the induction of HSV IE expression, Vmw65 forms a complex with TRF by recognition of the specific subset of appropriately flanked TRF binding sites present in each of the IE genes. This mechanism may provide a paradigm for the selective utilization of the same transcription factor in differential gene expression.

MeSH Terms
Base Sequence DNA/genetics,metabolism DNA-Binding Proteins/genetics Gene Expression Regulation Gene Products, tat Genes, Regulator Genes, Viral Humans Promoter Regions, Genetic Shelterin Complex Simplexvirus/genetics,metabolism Telomere-Binding Proteins Transcription Factors/genetics Transcription, Genetic
Chemicals
DNA-Binding Proteins Gene Products, tat Shelterin Complex TERF1 protein, human Telomere-Binding Proteins Transcription Factors DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
O'Hare P
Marie Curie Research Institute, Surrey, UK.
Goding C R
Haigh A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-12-20
Pages
4231-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC455136
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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