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PMID: 8035813 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sequential induction of NF-kappa B/Rel family proteins during B-cell terminal differentiation.

Molecular and cellular biology ·Vol. 14 ·No. 8 ·1994-08-00 ·Pages 5349-59

Liou HC, Sha WC, Scott ML, Baltimore D

Abstract

The NF-kappa B/Rel family of at least five transcription factor polypeptides is thought to function both as a developmental regulator in B cells and as a rapid response system in all cells. To examine this notion in more detail, we determined the protein contents of both the inducible and constitutive NF-kappa B/Rel activities in a pre-B-cell line, 70Z/3, and a mature B-cell line, WEHI 231. NF-kappa B p50/p65 is the major inducible nuclear complex after lipopolysaccharide or phorbol myristate acetate treatment of 70Z/3 cells. The constitutive and inducible complexes in WEHI 231 cells are mainly composed of p50 and Rel. The constitutive or induced activities are all sensitive to I kappa B-alpha, but this inhibitor is very short-lived in WEHI 231 cells, suggesting that the balance between synthesis and degradation of I kappa B-alpha determines whether a particular cell lineage has constitutive activity. A patterned expression of the NF-kappa B/Rel activator proteins emerges from an analysis of other B-lineage cell lines and splenic B cells: mainly p50 and p65 in pre-B (and non-B) cells, a predominance of Rel and p50 in mature B cells, and expression of p52 and RelB in plasmacytoma lines. This ordered pattern of regulators may reflect the requirement for expression of different genes during terminal B-cell differentiation because different combinations of NF-kappa B/Rel family members preferentially activate distinct kappa B sites in reporter constructs.

Related Genes
Rel myc
MeSH Terms
Animals B-Lymphocytes/cytology Base Sequence Cell Differentiation Cell Line Gene Expression Regulation Genes, Immunoglobulin Genes, myc Interferon-beta/genetics Mice Molecular Sequence Data Multigene Family NF-kappa B/metabolism Promoter Regions, Genetic Proto-Oncogene Proteins/metabolism RNA, Messenger/genetics Receptors, Interleukin-2/genetics Transcription Factor RelA Transcription Factor RelB Transcription Factors
Chemicals
NF-kappa B Proto-Oncogene Proteins RNA, Messenger Receptors, Interleukin-2 Relb protein, mouse Transcription Factor RelA Transcription Factors Transcription Factor RelB Interferon-beta
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Liou H C
Rockfeller University, New York, New York 10021.
Sha W C
Scott M L
Baltimore D
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.8.5349-5359.1994
Published
1994-08-00
Pages
5349-59
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359054
Subset
IM
Grants
PHS HHS · A108724-02 · United States
NCI NIH HHS · CA51462 · United States
NIGMS NIH HHS · GM39458 · United States
External Links
PubMed source DOI: 10.1128/mcb.14.8.5349-5359.1994 PMC Full Text View journal details
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