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PMID: 1634604 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Nuclear expression of the 50- and 65-kD Rel-related subunits of nuclear factor-kappa B is differentially regulated in human monocytic cells.

The Journal of clinical investigation ·Vol. 90 ·No. 1 ·1992-07-00 ·Pages 121-9

Kaufman PA, Weinberg JB, Greene WC

Abstract

The nuclear factor (NF)-kappa B transcription factor system is composed of at least four inducible nucleoprotein adducts termed p50, p55 (NF-kappa B p50), p75 (NF-kappa B p65), and p85 (c-Rel). These proteins are expressed in the nuclei of activated T cells in a distinctly biphasic fashion, with p55 and p75 induction occurring within minutes whereas the induction of p50 and p85 occurs after several hours. In contrast, p50 and p55 are constitutively expressed in the nuclei of U937 and THP-1 monocytic cells. However, cellular activation is required for the nuclear expression of p75 in these cells. Additionally, activation of monocytic cells does not result in a significant induction of p85. Tumor necrosis factor alpha induces the nuclear expression of p55 and p75 in these monocytic cells within 20 min, presumably reflecting the liberation of these proteins from I kappa B. In contrast, phorbol myristate acetate (PMA) induces the expression of these proteins with delayed kinetics, raising the possibility that PMA is incapable of mediating the efficient release of p55 and p75 from I kappa B in these cells. These findings highlight important differences in the regulation of these proteins in monocytic cells versus T cells and suggest that the induced expression of NF-kappa B p65 in monocytes may play a central role in the activation of HIV-1 gene expression.

MeSH Terms
Base Sequence Cell Line Cell Nucleus/chemistry Enhancer Elements, Genetic Gene Expression Regulation HIV Long Terminal Repeat Humans Molecular Sequence Data NF-kappa B/analysis Proto-Oncogene Proteins/analysis Proto-Oncogene Proteins c-rel Tetradecanoylphorbol Acetate/pharmacology Tumor Necrosis Factor-alpha/biosynthesis
Chemicals
NF-kappa B Proto-Oncogene Proteins Proto-Oncogene Proteins c-rel Tumor Necrosis Factor-alpha Tetradecanoylphorbol Acetate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kaufman P A
Howard Hughes Medical Institute, Durham, North Carolina.
Weinberg J B
Greene W C
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI115824
Published
1992-07-00
Pages
121-9
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC443070
Subset
IM
Grants
NCI NIH HHS · 5 KII CA01458-02 · United States
External Links
PubMed source DOI: 10.1172/JCI115824 PMC Full Text View journal details
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