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PMID: 803953 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Effect of lysogeny on transfection and transfection enhancement in Bacillus subtilis.

Journal of bacteriology ·Vol. 121 ·No. 1 ·1975-01-00 ·Pages 305-12

Yasbin RE, Wilson GA, Young FE

Abstract

Strains of Bacillus subtilis 168 lysogenic for bacteriophage phi105 transfer with deoxyribonucleic acid (DNA) isolated from bacteriophage SPO2 at a higher efficiency than non-lysogenic strains. This enhancement of transfection was not the result of recombination between bacteriophages SPO2 and phi105. Superinfection marker rescue increased transfection with DNA from bacteriophage phi105 occurred simultaneously with the addition of the transfecting DNA. Again, this enhancement of transfection was not the result of recombination but rather a protection of the transfecting DNA by the superinfecting bacteriophage. The ability of the superinfecting bacteriophage to protect the transfecting DNA from inactivation was maximal when the bacteria were just becoming competent. Bacteriophage phi1 cannot replicate after the transfection of competent bacteria lacking a functional DNA replication system, whereas bacteriophage phi1 was able to replicate after infection of competent bacteria grown under comparable conditions. These observations support the hypothesis that GAPase and an inducible repair system play an important role in the development of competence.

MeSH Terms
Azo Compounds Bacillus subtilis/enzymology,metabolism Bacteriophages/growth & development DNA Repair DNA Replication DNA Viruses DNA, Bacterial/biosynthesis DNA, Viral Enzyme Induction Enzyme Repression Lysogeny Models, Biological Transformation, Genetic Uracil/analogs & derivatives,pharmacology Virus Replication/drug effects
Chemicals
Azo Compounds DNA, Bacterial DNA, Viral Uracil
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Yasbin R E
Wilson G A
Young F E
References (42)
42 references, click to expand
  1. Regulation of the bacterial cell wall: analysis of a mutant of Bacillus subtilis defective in biosynthesis of teichoic acid.
    J Bacteriol. 1972 Apr;110(1):281-90 PMID: 4622900
  2. Involvement of recA and exr genes in the in vivo inhibition of the recBC nuclease.
    J Bacteriol. 1974 May;118(2):465-70 PMID: 4597445
  3. Some properties of ATP dependent deoxyribonucleases from normal and rec-mutant strains of Bacillus subtilis.
    FEBS Lett. 1972 Jul 15;24(1):121-5 PMID: 4628739
  4. Genetic transformation in Escherichia coli K12.
    Proc Natl Acad Sci U S A. 1973 Jan;70(1):84-7 PMID: 4630612
  5. The effect of bacteriophage T4 infection on an ATP-dependent deoxyribonuclease in Escherichia coli.
    Biochim Biophys Acta. 1971 Feb 11;228(3):767-9 PMID: 4929432
  6. Transfection enhancement by ultraviolet irradiation.
    Biochem Biophys Res Commun. 1967 Apr 20;27(2):258-62 PMID: 4962317
  7. Evidence that Bacillus subtilis bacteriophage SP02 is temperate and heteroimmune to bacteriophage phi-105.
    J Virol. 1969 Jul;4(1):47-9 PMID: 4980071
  8. Some properties of DNA in competent Bacillus subtilis.
    J Mol Biol. 1969 Jan;39(2):245-55 PMID: 4983063
  9. Inhibition of bacterial DNA replication by 6-(p-hydroxyphenylazo)-uracil: differential effect on repair and semi-conservative synthesis in Bacillus subtilis.
    J Mol Biol. 1971 Jul 14;59(1):1-16 PMID: 4997657
  10. The role of recombination in transfection of B. subtilis.
    Mol Gen Genet. 1971;113(2):174-90 PMID: 5002582
  11. Structural features of DNA in competent Bacillus subtilis.
    Mol Gen Genet. 1971;113(4):316-30 PMID: 5004185
  12. Growth of bacteriophage phi 105 and its deoxyribonucleic acid in radiation-sensitive mutants of Bacillus subtilis.
    J Virol. 1971 Dec;8(6):919-21 PMID: 5006010
  13. INFECTIOUS DNA FROM A NEWLY ISOLATED B. SUBTILIS PHAGE.
    Z Vererbungsl. 1964 Apr 10;95:57-65 PMID: 14196020
  14. INFECTIVITY OF DNA ISOLATED FROM BACILLUS SUBTILIS BACTERIOPHAGE, SP82.
    J Mol Biol. 1964 Dec;10:438-51 PMID: 14255111
  15. BACTERIOPHAGE DEOXYRIBONUCLEATE INFECTION OF COMPETENT BACILLUS SUBTILIS.
    J Bacteriol. 1965 Mar;89:782-90 PMID: 14273661
  16. Fate of transforming DNA following uptake by competent Bacillus subtilis. Formation and properties of products isolated from transformed cells which are derived entirely from donor DNA.
    J Mol Biol. 1972 Feb 28;64(1):9-29 PMID: 4622632
  17. Bacteriophage SP82G inhibition of an intracellular deoxyribonucleic acid inactivation process in Bacillus subtilis.
    J Virol. 1972 Jul;10(1):51-9 PMID: 4625174
  18. Inhibition of a DNA polymerase from Bacillus subtilis by hydroxyphenylazopyrimidines.
    Proc Natl Acad Sci U S A. 1973 Jan;70(1):103-7 PMID: 4630611
  19. Transformation and transfection in lysogenic strains of Bacillus subtilis 168.
    J Bacteriol. 1973 Feb;113(2):540-8 PMID: 4632315
  20. Mechanisms of enhancement of SP82 transfection.
    J Virol. 1968 Jul;2(7):710-5 PMID: 4972781
  21. Mechanism of transfection with deoxyribonucleic acid from the temperate Bacillus bacteriophage phi-105.
    J Virol. 1969 Jul;4(1):50-7 PMID: 4980072
  22. Fate of transforming DNA following uptake by competent Bacillus subtilis. I. Formation and properties of the donor-recipient complex.
    J Mol Biol. 1971 Mar 14;56(2):209-21 PMID: 4994568
  23. Source of the nonlinear dependence of bacteriophage SP82 transfection on deoxyribonucleic acid concentration.
    J Virol. 1971 Jun;7(6):749-52 PMID: 4998320
  24. Mechanism of transformation in B. subtilis.
    Mol Gen Genet. 1971;113(4):331-44 PMID: 5004186
  25. Ultraviolet reactivation and ultraviolet mutagenesis of lambda in different genetic systems.
    Virology. 1971 Feb;43(2):495-503 PMID: 5543835
  26. THE POSSIBLE ROLE OF RECOMBINATION IN THE INFECTION OF COMPETENT BACILLUS SUBTILIS BY BACTERIOPHAGE DEOXYRIBONUCLEIC ACID.
    Virology. 1964 Dec;24:552-62 PMID: 14240402
  27. Recombination and transfection mapping of cistron 5 of bacteriophage sp82g.
    Genetics. 1972 Feb;70(2):187-203 PMID: 17248556
  28. Transformation and transfection in lysogenic strains of Bacillus subtilis: evidence for selective induction of prophage in competent cells.
    J Bacteriol. 1975 Jan;121(1):296-304 PMID: 803952
  29. Recombination deficient mutants of E. coli and other bacteria.
    Annu Rev Genet. 1973;7:67-86 PMID: 4205905
  30. Bacteriophage interference in Bacillus subtilis 168.
    J Virol. 1974 Apr;13(4):916-21 PMID: 4206943
  31. Transfection of Bacillus subtilis with bacteriophage H1 DNA: fate of transfecting DNA and transfection enhancement in B. subtilis uur+ and uur- strains.
    Mol Gen Genet. 1974;128(1):55-72 PMID: 4207204
  32. Transfection in B. subtilis.
    Curr Top Microbiol Immunol. 1973;62:61-88 PMID: 4208299
  33. ATP-dependent deoxyribonuclease in Bacillus subtilis and a mutant deficient in this activity.
    Mutat Res. 1974 Jan;22(1):15-23 PMID: 4210325
  34. The recBC deoxyribonuclease of Escherichia coli K-12. Substrate specificity and reaction intermediates.
    J Biol Chem. 1973 Jul 25;248(14):4874-84 PMID: 4268693
  35. Factors affecting competence for transformation in Staphylococcus aureus.
    J Bacteriol. 1974 Apr;118(1):155-64 PMID: 4274456
  36. Purification and properties of the gamma-protein specified by bacteriophage lambda: an inhibitor of the host RecBC recombination enzyme.
    Proc Natl Acad Sci U S A. 1973 Aug;70(8):2215-9 PMID: 4275917
  37. Inhibitory effect of bacteriophage P22 infection on host cell deoxyribonuclease activity.
    J Virol. 1972 May;9(5):752-7 PMID: 4554464
  38. The genetic and biochemical basis of the transformability of Escherichia coli K12.
    Biochem Biophys Res Commun. 1972 Dec 18;49(6):1568-72 PMID: 4565378
  39. Ultraviolet mutagenesis in polA and UvrA polA derivatives of Escherichia coli B-R: evidence for an inducible error-prone repair system.
    Genetics. 1973 Apr;73:Suppl 73:91-10 PMID: 4576123
  40. The nature of the transformation process in Escherichia coli K12.
    Mol Gen Genet. 1973 Jul 31;124(1):1-10 PMID: 4582298
  41. Indirect ultraviolet-reactivation of phage lambda.
    Proc Natl Acad Sci U S A. 1974 Jan;71(1):144-7 PMID: 4589889
  42. Unrelatedness of temperate Bacillus subtilis bacteriophages SP02 and phi105.
    J Virol. 1972 May;9(5):732-7 PMID: 4623614
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.121.1.305-312.1975
Published
1975-01-00
Pages
305-12
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC285644
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.121.1.305-312.1975 PMC Full Text View journal details
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