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PMID: 8045913 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Molecular cloning of two new heat shock genes related to the hsp70 genes in Staphylococcus aureus.

Journal of bacteriology ·Vol. 176 ·No. 15 ·1994-08-00 ·Pages 4779-83

Ohta T, Saito K, Kuroda M, Honda K, Hirata H, Hayashi H

Abstract

We have identified two new heat shock protein genes, orf37 and orf35, in Staphylococcus aureus, located upstream and downstream of grpE(hsp20), dnaK(hsp70), and dnaJ(hsp40) homologous genes in the order orf37-hsp20-hsp70-hsp40-orf35. The transcripts of both orf37 and orf35 were increased by thermal upshift of the culture from 37 to 46 degrees C. The heat shock promoters were located upstream of orf37 and upstream of hsp40. The deduced peptide of orf37 showed similarity with those of orfA in Clostridium acetobutylicum and orf39 in Bacillus subtilis. orf35 was unique in S. aureus and has not yet been described in other bacteria.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Electrophoresis, Gel, Two-Dimensional Genes, Bacterial/genetics Heat-Shock Proteins/biosynthesis,genetics Molecular Sequence Data Restriction Mapping Sequence Analysis, DNA Sequence Homology, Amino Acid Staphylococcus aureus/genetics
Chemicals
Heat-Shock Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ohta T
Department of Microbiology, University of Tsukuba, Ibaraki, Japan.
Saito K
Kuroda M
Honda K
Hirata H
Hayashi H
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32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.15.4779-4783.1994
Published
1994-08-00
Pages
4779-83
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC196305
Subset
IM
Databases
GENBANK
D30690
External Links
PubMed source DOI: 10.1128/jb.176.15.4779-4783.1994 PMC Full Text View journal details
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