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PMID: 8096841 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning, sequencing, and transcriptional analysis of the groESL operon from Bacillus stearothermophilus.

Journal of bacteriology ·Vol. 175 ·No. 8 ·1993-04-00 ·Pages 2465-9

Schön U, Schumann W

Abstract

Using a gene probe of the Bacillus subtilis groEL gene, a 7.3-kb HindIII fragment of chromosomal DNA of Bacillus stearothermophilus was cloned. Sequencing of 2,309 bp led to the detection of two open reading frames in the order groES groEL. Primer extension studies revealed one potential transcription start site preceding the groESL operon, which was activated upon temperature upshift. Northern blot (RNA) analysis resolved two mRNA species with lengths of 2.2 and 1.5 kb; RNA slot-blot experiments revealed an at least 10-fold increase in the amount of specific mRNA from 0 to 7 min postinduction followed by a decrease. The 9-bp inverted repeat characteristic of many gram-positive bacteria was found within the 5' leader region of the mRNA. The groESL operon of B. stearothermophilus could complement E. coli groES(Ts) and groEL(Ts) mutants for growth at high temperature and for propagation of phage lambda.

Related Genes
groEL groES
MeSH Terms
Amino Acid Sequence Bacterial Proteins/analysis,chemistry,genetics Base Sequence Chaperonin 10 Chaperonin 60 Cloning, Molecular Genes, Bacterial Geobacillus stearothermophilus/genetics Heat-Shock Proteins/analysis,chemistry,genetics Molecular Sequence Data Operon Transcription, Genetic
Chemicals
Bacterial Proteins Chaperonin 10 Chaperonin 60 Heat-Shock Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schön U
Lehrstuhl für Genetik, Universität Bayreuth, Germany.
Schumann W
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34 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.8.2465-2469.1993
Published
1993-04-00
Pages
2465-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC204540
Subset
IM
Databases
GENBANK
L10132
External Links
PubMed source DOI: 10.1128/jb.175.8.2465-2469.1993 PMC Full Text View journal details
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