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PMID: 1350777 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning, sequencing, mapping, and transcriptional analysis of the groESL operon from Bacillus subtilis.

Journal of bacteriology ·Vol. 174 ·No. 12 ·1992-06-00 ·Pages 3993-9

Schmidt A, Schiesswohl M, Völker U, Hecker M, Schumann W

Abstract

Using a gene probe of the Escherichia coli groEL gene, a 1.8-kb HindIII fragment of chromosomal DNA of Bacillus subtilis was cloned. Upstream sequences were isolated as a 3-kb PstI fragment. Sequencing of 2,525 bp revealed two open reading frames in the order groES groEL. Alignment of the GroES and GroEL proteins with those of eight other eubacteria revealed 50 to 65% and 72 to 84% sequence similarity, respectively. Primer extension studies revealed one potential transcription start site preceding the groESL operon (S) which was activated upon temperature upshift. Northern (RNA) analysis led to the detection of two mRNA species of 2.2 and 1.5 kb. RNA dot blot experiments revealed an at least 10-fold increase in the amount of specific mRNA from 0 to 5 min postinduction, remaining at this high level for 10 min and then decreasing. A 9-bp inverted repeat within the 5' leader region of the mRNA might be involved in regulation of the heat shock response. By using PBS1 transduction, the groESL operon was mapped at about 342 degrees.

Related Genes
groEL groES
MeSH Terms
Amino Acid Sequence Bacillus subtilis/genetics Bacterial Proteins/chemistry,genetics Base Sequence Blotting, Northern Chaperonin 10 Chaperonin 60 Cloning, Molecular Heat-Shock Proteins/chemistry,genetics Molecular Sequence Data Operon/genetics Plasmids/genetics Restriction Mapping Transcription, Genetic/genetics
Chemicals
Bacterial Proteins Chaperonin 10 Chaperonin 60 Heat-Shock Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Schmidt A
Lehrstuhl für Genetik, Universität Bayreuth, Germany.
Schiesswohl M
Völker U
Hecker M
Schumann W
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.12.3993-3999.1992
Published
1992-06-00
Pages
3993-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206108
Subset
IM
Databases
GENBANK
M79367, M79368, M79369, M79370, M79371, M79372, M84965, M84973, M84974, M84980
External Links
PubMed source DOI: 10.1128/jb.174.12.3993-3999.1992 PMC Full Text View journal details
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