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PMID: 8053494 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Organ site-dependent expression of basic fibroblast growth factor in human renal cell carcinoma cells.

The American journal of pathology ·Vol. 145 ·No. 2 ·1994-08-00 ·Pages 365-74

Singh RK, Bucana CD, Gutman M, Fan D, Wilson MR, Fidler IJ

Abstract

We investigated the influence of organ microenvironment on the angiogenic phenotype in human renal cell carcinoma (HRCC) cells. HRCC line SN12C was established in vitro from a surgical specimen, and metastatic line SN12PM6 was isolated from a lung metastasis produced by parental cells implanted into the kidney of nude mice. SN12C (low metastasis) and SN12PM6 (high metastasis) cells were injected into the kidney or subcutis of nude mice. The kidney tumors were highly vascularized (as revealed by immunohistochemistry using antibodies against factor VIII), and metastatic, whereas the subcutaneous tumors were not. The expression of mRNA for basic fibroblast growth factor (bFGF) in kidney tumors was 10 to 20 times that found in subcutaneous tumors. Similar data were obtained at the protein level by using fluorescence activated cell sorting, immunohistochemistry, and enzyme-linked immunosorbent assay. bFGF was detected in the urine of mice with tumors in the kidney but not subcutaneous tumors. The level of bFGF in the serum of mice with kidney tumors was two to three times that in mice with subcutaneous tumors. The changes in bFGF expression in the tumors was transient. Collectively, these data indicate that the organ microenvironment can influence the expression level of bFGF in HRCC.

MeSH Terms
Animals Carcinoma, Renal Cell/metabolism,pathology Fibroblast Growth Factor 2/metabolism Humans Kidney Neoplasms/metabolism,pathology Male Mice Mice, Nude Neoplasm Transplantation Skin Neoplasms/metabolism Tissue Distribution Tumor Cells, Cultured
Chemicals
Fibroblast Growth Factor 2
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Singh R K
Department of Cell Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Bucana C D
Gutman M
Fan D
Wilson M R
Fidler I J
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1994-08-00
Pages
365-74
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1887407
Subset
IM
Grants
NCI NIH HHS · CA 16672 · United States
NCI NIH HHS · R35-CA-42107 · United States
External Links
PubMed source PMC Full Text View journal details
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