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PMID: 8065337 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mutation of the gene encoding protein kinase C 1 stimulates mitotic recombination in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 14 ·No. 9 ·1994-09-00 ·Pages 6039-45

Huang KN, Symington LS

Abstract

We have isolated a recessive allele of the yeast protein kinase C gene (PKC1) which promotes an elevated rate of mitotic recombination and confers a temperature-sensitive growth defect. The rate of recombination was increased between genes in direct repeat and at a series of heteroalleles and was dependent upon the RAD52 gene product. The mutant pkc1 allele was sequenced and found to encode a single amino acid change within the catalytic domain. Osmotic stabilizing agents rescued the temperature-sensitive growth defect but not the hyperrecombination phenotype, indicating that the two traits are separable. This separability suggests that the PKC1 gene product (Pkc1p) regulates DNA metabolism by an alternate pathway to that used in the regulation of cell lysis. The regulation of recombination is a previously unidentified role for Pkc1p.

Related Genes
PKC1 RAD1 RAD52
MeSH Terms
Alleles Amino Acid Sequence DNA-Binding Proteins/physiology Fungal Proteins/physiology Gene Conversion Isoenzymes/chemistry,physiology Mitosis Molecular Sequence Data Mutation Protein Kinase C/physiology Rad52 DNA Repair and Recombination Protein Recombination, Genetic Saccharomyces cerevisiae/enzymology,genetics Saccharomyces cerevisiae Proteins Sequence Alignment Sequence Homology, Amino Acid
Chemicals
DNA-Binding Proteins Fungal Proteins Isoenzymes RAD52 protein, S cerevisiae Rad52 DNA Repair and Recombination Protein Saccharomyces cerevisiae Proteins Protein Kinase C
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Huang K N
Institute of Cancer Research, Columbia University College of Physicians and Surgeons, New York, New York 10032.
Symington L S
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.9.6039-6045.1994
Published
1994-09-00
Pages
6039-45
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359130
Subset
IM
Grants
NIGMS NIH HHS · R01 GM041784 · United States
NCI NIH HHS · CA09503 · United States
NIGMS NIH HHS · GM41784 · United States
External Links
PubMed source DOI: 10.1128/mcb.14.9.6039-6045.1994 PMC Full Text View journal details
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