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PMID: 8070409 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A member of a novel family of yeast 'zn-finger' proteins mediates the transition from stationary phase to cell proliferation.

The EMBO journal ·Vol. 13 ·No. 16 ·1994-08-15 ·Pages 3812-21

Ireland LS, Johnston GC, Drebot MA, Dhillon N, DeMaggio AJ, Hoekstra MF, Singer RA

Abstract

The cloning and molecular characterization of the GCS1 gene from the budding yeast Saccharomyces cerevisiae show that stationary phase is in fact a unique developmental state, with requirements to resume cell proliferation that can be distinct from those for maintenance of proliferation. Deletion of the GCS1 gene products a novel phenotype: stationary-phase mutant cells do not resume proliferation at a restrictive temperature of 15 degrees C, but mutant cells lacking Gcs1p that are proliferating at the permissive temperature of 29 degrees C continue to proliferate after transfer to 15 degrees C as long as nutrients are available. The GCS1 gene sequence predicts a 39 kDa polypeptide with a novel 'Zn-finger' motif. A point mutation within the finger motif produces a phenotype that mimics that of deletion of the GCS1 gene, showing that the finger motif is essential for full Gcs1p activity. Gcs1p and the products of two newly identified genes, SPS18 and GLO3, constitute a family of novel Zn-finger proteins.

Related Genes
GCS1
MeSH Terms
Amino Acid Sequence Base Sequence Cell Division/genetics Culture Media DNA-Binding Proteins/genetics Fungal Proteins/genetics GTPase-Activating Proteins Genes, Fungal/genetics Genetic Complementation Test Molecular Sequence Data Multigene Family/genetics Mutation Phenotype RNA, Messenger/genetics Recombinant Fusion Proteins Restriction Mapping Saccharomyces cerevisiae/genetics,growth & development,physiology Saccharomyces cerevisiae Proteins Sequence Analysis, DNA Sequence Homology, Amino Acid Structure-Activity Relationship Zinc Fingers/genetics
Chemicals
Culture Media DNA-Binding Proteins Fungal Proteins GCS1 protein, S cerevisiae GTPase-Activating Proteins Glo3 protein, S cerevisiae RNA, Messenger Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ireland L S
Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia, Canada.
Johnston G C
Drebot M A
Dhillon N
DeMaggio A J
Hoekstra M F
Singer R A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-08-15
Pages
3812-21
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395294
Subset
IM
Databases
GENBANK
L24125, X79514
External Links
PubMed source PMC Full Text View journal details
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