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PMID: 8071194 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

In vivo footprinting analysis of Lrp binding to the ilvIH promoter region of Escherichia coli.

Journal of bacteriology ·Vol. 176 ·No. 17 ·1994-09-00 ·Pages 5197-201

Marasco R, Varcamonti M, La Cara F, Ricca E, De Felice M, Sacco M

Abstract

An in vivo footprinting analysis of the ilvIH regulatory region of Escherichia coli showed that the transcription activator Lrp binds to six sites, scattered over 250 bp upstream of the transcriptional start point. When Lrp-mediated activation was impaired by the presence of exogenous leucine, only one promoter-distal site (site 2) was partially protected by Lrp binding. Equilibrium dialysis experiments showed the formation of an Lrp-leucine complex in vitro. These results suggest that leucine negatively affects ilvIH transcription because its interaction with Lrp reduces the efficiency of binding of the regulatory protein to the promoter region.

Related Genes
ilvI livH
MeSH Terms
Bacterial Proteins/metabolism Base Sequence Binding Sites DNA, Bacterial/genetics,isolation & purification,metabolism DNA-Binding Proteins/metabolism Escherichia coli/genetics,metabolism Escherichia coli Proteins Kinetics Leucine/metabolism Leucine-Responsive Regulatory Protein Methylation Molecular Sequence Data Operon Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid Transcription Factors/metabolism
Chemicals
Bacterial Proteins DNA, Bacterial DNA-Binding Proteins Escherichia coli Proteins Lrp protein, E coli Transcription Factors Leucine-Responsive Regulatory Protein Leucine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Marasco R
Dipartimento di Fisiologia Generale ed Ambientale, Università Federico II, Naples, Italy.
Varcamonti M
La Cara F
Ricca E
De Felice M
Sacco M
References (26)
26 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.17.5197-5201.1994
Published
1994-09-00
Pages
5197-201
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC196701
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.176.17.5197-5201.1994 PMC Full Text View journal details
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