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PMID: 6168634 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Physical characterization of the ilvHI operon of Escherichia coli K-12.

Journal of bacteriology ·Vol. 147 ·No. 3 ·1981-09-00 ·Pages 797-804

Squires CH, De Felice M, Wessler SR, Calvo JM

Abstract

The ilvHI and leu genes of Escherichia coli K-12 are contained on a single 10.9-kilobase EcoRI fragment of deoxyribonucleic acid derived from the leu transducing phage lambda G4. Since the expression of all of these genes is controlled by leucine, we investigated whether they are part of single operon or whether they constitute separate but adjacent operons controlled from a common site. Both cloning and hybridization studies indicated that ilvHI and leu are distinct operons. They are transcribed in opposite directions and are separated by approximately 1,500 base pairs of deoxyribonucleic acid. Hybridization experiments showed that the expression of ilvHI is regulated chiefly at the level of transcription. The size of the ilvHI messenger ribonucleic acid is estimated to be 2,550 bases.

MeSH Terms
Acetolactate Synthase/genetics Cloning, Molecular Escherichia coli/genetics,metabolism Leucine/biosynthesis,pharmacology Nucleic Acid Hybridization Operon Oxo-Acid-Lyases/genetics RNA, Bacterial/metabolism RNA, Messenger/metabolism Transcription, Genetic
Chemicals
RNA, Bacterial RNA, Messenger Acetolactate Synthase Oxo-Acid-Lyases Leucine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Squires C H
De Felice M
Wessler S R
Calvo J M
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22 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.147.3.797-804.1981
Published
1981-09-00
Pages
797-804
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216115
Subset
IM
Grants
NIAID NIH HHS · AI 14340 · United States
External Links
PubMed source DOI: 10.1128/jb.147.3.797-804.1981 PMC Full Text View journal details
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