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PMID: 8076601 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Two distinct mechanisms contribute to the constitutive activation of RelB in lymphoid cells.

The EMBO journal ·Vol. 13 ·No. 17 ·1994-09-01 ·Pages 4060-9

Lernbecher T, Kistler B, Wirth T

Abstract

The kappa B-motif is an important regulatory element both for constitutive lymphoid-specific as well as ubiquitous inducible transcriptional activity. We have shown previously that different members of the NF-kappa B/Rel family of transcription factors are responsible for these distinct functions. Whereas the p65/RelA protein is involved in inducible kappa B-dependent transcription, RelB is associated with constitutive activity in lymphoid cells. Here we have addressed the question of how RelB is constitutively activated in lymphoid cells. We demonstrate that this is achieved by two different mechanisms. Expression of relB as determined by measurement of stable RNA and protein levels is significantly enhanced in lymphoid organs compared with non-lymphoid organs. However, these observed differences in absolute amounts of RNA and protein would not suffice to explain the dramatic differences that are apparent at the level of active DNA binding complexes in extracts from the respective organs. We have therefore analyzed the interaction of RelB complexes with the I kappa B-alpha inhibitor protein. Our results show that RelB-containing complexes present in lymphoid extracts are much less susceptible to inhibition by I kappa B-alpha than RelA- or RelB-containing complexes from non-lymphoid cells. This difference might be due to post-translational modifications of the RelB protein or interaction with a lymphoid-specific cofactor for RelB.

MeSH Terms
Animals DNA-Binding Proteins/metabolism I-kappa B Proteins Lymphoid Tissue/cytology,metabolism Mice NF-KappaB Inhibitor alpha NF-kappa B/metabolism Protein Binding Proto-Oncogene Proteins Regulatory Sequences, Nucleic Acid Tissue Distribution Transcription Factor RelA Transcription Factor RelB Transcription Factors/metabolism Transcription, Genetic
Chemicals
DNA-Binding Proteins I-kappa B Proteins NF-kappa B Nfkbia protein, mouse Proto-Oncogene Proteins Relb protein, mouse Transcription Factor RelA Transcription Factors NF-KappaB Inhibitor alpha Transcription Factor RelB
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lernbecher T
Zentrum für Molekulare Biologie Heidelberg, Germany.
Kistler B
Wirth T
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-09-01
Pages
4060-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395327
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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