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PMID: 8090744 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Adeno-associated virus vectors preferentially transduce cells in S phase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 19 ·1994-09-13 ·Pages 8915-9

Russell DW, Miller AD, Alexander IE

Abstract

Vectors based on adeno-associated virus can stably transfer genes by chromosomal integration in recipient cells. In this study we have infected stationary and dividing primary human fibroblast cultures with adeno-associated virus vectors encoding alkaline phosphatase and neomycin phosphotransferase. We find that the transduction frequency of S phase cells is about 200 times that of non-S phase cells. However, neither S phase nor mitosis is essential for transduction. Single-stranded vector genomes survive in stationary cultures and can be recruited for transduction by stimulating these cultures to divide. Stable transductants contain randomly integrated vector sequences. These findings have important implications for the use of adeno-associated virus vectors in gene therapy.

MeSH Terms
Alkaline Phosphatase Cells, Cultured Dependovirus/genetics Genetic Therapy/methods Genetic Vectors Humans In Vitro Techniques Plasmids S Phase Transduction, Genetic Virus Integration
Chemicals
Alkaline Phosphatase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Russell D W
Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
Miller A D
Alexander I E
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.19.8915
Published
1994-09-13
Pages
8915-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC44717
Subset
IM
Grants
NCI NIH HHS · CA09645 · United States
NHLBI NIH HHS · HL36444 · United States
NHLBI NIH HHS · HL41212 · United States
External Links
PubMed source DOI: 10.1073/pnas.91.19.8915 PMC Full Text View journal details
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