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PMID: 8097208 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

In vivo cytokine profiles in patients with kala-azar. Marked elevation of both interleukin-10 and interferon-gamma.

The Journal of clinical investigation ·Vol. 91 ·No. 4 ·1993-04-00 ·Pages 1644-8

Karp CL, el-Safi SH, Wynn TA, Satti MM, Kordofani AM, Hashim FA, Hag-Ali M, Neva FA, Nutman TB, Sacks DL

Abstract

The immunological mechanisms underlying the susceptibility to disseminated visceral parasitism of mononuclear phagocytes in patients with kala-azar remain undefined. Resistance and susceptibility are correlated with distinct patterns of cytokine production in murine models of disseminated leishmanial disease. To assess lesional cytokine profiles in patients with kala-azar, bone marrow aspirates were analyzed using a quantitative reverse transcriptase PCR technique to amplify specific mRNA sequences of multiple Th1-, Th2-, and/or macrophage-associated cytokines. Transcript levels of IL-10 as well as IFN-gamma were significantly elevated in patients with active visceral leishmaniasis; IL-10 levels decreased markedly with resolution of disease. These findings suggest that IL-10, a potent, pleiotropic suppressor of all known microbicidal effector functions of macrophages, may contribute to the pathogenesis of kala-azar by inhibiting the cytokine-mediated activation of host macrophages that is necessary for the control of leishmanial infection.

MeSH Terms
Adolescent Adult Base Sequence Bone Marrow/chemistry,pathology CD4-Positive T-Lymphocytes/physiology Child Cytokines/genetics,physiology Female Humans Interferon-gamma/analysis Interleukin-10/analysis Leishmaniasis, Visceral/metabolism,pathology Male Middle Aged Molecular Sequence Data Phenotype RNA, Messenger/analysis T-Lymphocyte Subsets/physiology
Chemicals
Cytokines RNA, Messenger Interleukin-10 Interferon-gamma
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Karp C L
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
el-Safi S H
Wynn T A
Satti M M
Kordofani A M
Hashim F A
Hag-Ali M
Neva F A
Nutman T B
Sacks D L
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI116372
Published
1993-04-00
Pages
1644-8
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC288142
Subset
IM
Grants
NIAID NIH HHS · 2PO1 AI 16312 · United States
Corrections
CommentIn
PMID: 8473479
External Links
PubMed source DOI: 10.1172/JCI116372 PMC Full Text View journal details
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