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PMID: 8105467 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

v-jun cooperates with v-erbB to transform the thrombocytic/megakaryocytic lineage.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 19 ·1993-10-01 ·Pages 8837-41

Garcia M, Samarut J

Abstract

The transforming properties of v-jun, the viral counterpart of the transcription factor AP1, were investigated in avian hematopoietic cells. Two retroviruses, called JB and JBN, expressing both v-jun and v-erbB, were constructed using an avian erythroblastosis-based vector. We show that the cooperative action of both oncogenes allowed the virus to efficiently transform bone marrow cells. No such transformation was obtained with either oncogene alone. JB-transformed bone marrow cells expressed GATA-1, TAL-1, and histone H5, suggesting that they belong to the erythrocytic/thrombocytic lineage. (Thrombocytes are the avian homologues of mammal megakaryocytes.) Moreover, after induction with phorbol 12-myristate 13-acetate JB-transformed bone marrow cells began to differentiate and synthesized high levels of platelet glycoproteins, indicating that they were of thrombocytic origin. These results were confirmed by c-ets1 analysis since this transcription factor, specifically found in cells with megakaryocytic but not erythrocytic features, was clearly detected in these cells.

Related Genes
c-ets1 gapdh v-erbB v-jun
MeSH Terms
Animals Base Sequence Blood Platelets/cytology,metabolism Bone Marrow/drug effects Bone Marrow Cells Cell Differentiation/drug effects Cell Line, Transformed Cells, Cultured Chick Embryo Chickens DNA Primers Fibroblasts Genes, jun Globins/genetics Glyceraldehyde-3-Phosphate Dehydrogenases/genetics Hematopoietic Stem Cells/cytology,metabolism Histones/analysis,biosynthesis,isolation & purification Megakaryocytes/cytology,metabolism Molecular Sequence Data Oncogene Protein p65(gag-jun)/genetics Oncogene Proteins v-erbB Oncogenes Platelet Membrane Glycoproteins/analysis,biosynthesis,isolation & purification Polymerase Chain Reaction Proto-Oncogene Protein c-ets-1 Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-ets Proto-Oncogenes Retroviridae Proteins, Oncogenic/genetics Tetradecanoylphorbol Acetate/pharmacology Transcription Factors
Chemicals
DNA Primers Histones Oncogene Protein p65(gag-jun) Oncogene Proteins v-erbB Platelet Membrane Glycoproteins Proto-Oncogene Protein c-ets-1 Proto-Oncogene Proteins Proto-Oncogene Proteins c-ets Retroviridae Proteins, Oncogenic Transcription Factors Globins Glyceraldehyde-3-Phosphate Dehydrogenases Tetradecanoylphorbol Acetate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Garcia M
Ecole Normale Supérieure de Lyon, Laboratoire de Biologie Moléculaire et Cellulaire, Institut National de la Recherche Agronomique, France.
Samarut J
References (45)
45 references, click to expand
  1. Transforming capacities of avian erythroblastosis virus mutants deleted in the erbA or erbB oncogenes.
    Cell. 1983 Jan;32(1):227-38 PMID: 6297784
  2. Expression of an erythroid transcription factor in megakaryocytic and mast cell lineages.
    Nature. 1990 Mar 29;344(6265):444-7 PMID: 2320112
  3. Expression of platelet membrane glycoproteins and alpha-granule proteins by a human erythroleukemia cell line (HEL).
    EMBO J. 1984 Feb;3(2):453-9 PMID: 6201359
  4. Synthesis of analogs of human platelet membrane glycoprotein IIb-IIIa complex by chicken peripheral blood thrombocytes.
    Proc Natl Acad Sci U S A. 1985 Nov;82(21):7319-23 PMID: 2932737
  5. A new site of integration for mouse mammary tumor virus proviral DNA common to BALB/cf(C3H) mammary and kidney adenocarcinomas.
    EMBO J. 1986 Jan;5(1):127-34 PMID: 3007110
  6. v-erbA cooperates with sarcoma oncogenes in leukemic cell transformation.
    Cell. 1986 May 9;45(3):349-56 PMID: 3009024
  7. The c-erb-A protein is a high-affinity receptor for thyroid hormone.
    Nature. 1986 Dec 18-31;324(6098):635-40 PMID: 2879242
  8. The c-erb-A gene encodes a thyroid hormone receptor.
    Nature. 1986 Dec 18-31;324(6098):641-6 PMID: 2879243
  9. The in vitro synthesis of polypeptides for the platelet membrane glycoproteins IIb and IIIa.
    Blood. 1987 Apr;69(4):1031-7 PMID: 3548842
  10. Homology between the DNA-binding domain of the GCN4 regulatory protein of yeast and the carboxyl-terminal region of a protein coded for by the oncogene jun.
    Proc Natl Acad Sci U S A. 1987 May;84(10):3316-9 PMID: 3554236
  11. Regulated expression of multiple chicken erythroid membrane skeletal protein 4.1 variants is governed by differential RNA processing and translational control.
    Proc Natl Acad Sci U S A. 1987 Jul;84(13):4432-6 PMID: 3474611
  12. Human proto-oncogene c-jun encodes a DNA binding protein with structural and functional properties of transcription factor AP-1.
    Science. 1987 Dec 4;238(4832):1386-92 PMID: 2825349
  13. Oncogene jun encodes a sequence-specific trans-activator similar to AP-1.
    Nature. 1988 Mar 10;332(6160):166-71 PMID: 3347253
  14. Rapid transformation of cells by Rous sarcoma virus.
    Proc Natl Acad Sci U S A. 1969 Jun;63(2):318-25 PMID: 4308272
  15. K562--a human erythroleukemic cell line.
    Int J Cancer. 1979 Feb;23(2):143-7 PMID: 367973
  16. Comparative studies on chromatin proteins from chicken thrombocytes and erythrocytes.
    Biochim Biophys Acta. 1980 Aug 21;624(2):522-30 PMID: 7417490
  17. The formation of Ca++-dependent complexes of platelet membrane glycoproteins IIb and IIIa in solution as determined by crossed immunoelectrophoresis.
    Blood. 1981 Aug;58(2):268-78 PMID: 6454451
  18. Complex formation of platelet membrane glycoproteins IIb and IIIa with fibrinogen.
    J Clin Invest. 1982 Feb;69(2):263-9 PMID: 6460044
  19. Isolation and characterization of chicken DNA homologous to the two putative oncogenes of avian erythroblastosis virus.
    Cell. 1982 Jan;28(1):135-43 PMID: 6279309
  20. HEL cells: a new human erythroleukemia cell line with spontaneous and induced globin expression.
    Science. 1982 Jun 11;216(4551):1233-5 PMID: 6177045
  21. Megakaryocytic and erythrocytic lineages share specific transcription factors.
    Nature. 1990 Mar 29;344(6265):447-9 PMID: 2320113
  22. v-erbA oncogene activation entails the loss of hormone-dependent regulator activity of c-erbA.
    Cell. 1990 Jun 15;61(6):1035-49 PMID: 1972036
  23. Cooperation of v-jun and v-erbB oncogenes in embryo fibroblast transformation in vitro and in vivo.
    J Virol. 1990 Oct;64(10):4684-90 PMID: 1975843
  24. Amplified expression of three jun family members inhibits erythroleukemia differentiation.
    Blood. 1990 Nov 1;76(9):1830-7 PMID: 2121297
  25. The carbonic anhydrase II gene, a gene regulated by thyroid hormone and erythropoietin, is repressed by the v-erbA oncogene in erythrocytic cells.
    New Biol. 1990 Mar;2(3):284-94 PMID: 2126201
  26. Erythroid expression of the 'helix-loop-helix' gene, SCL.
    Oncogene. 1991 Mar;6(3):475-9 PMID: 2011404
  27. Cross-family dimerization of transcription factors Fos/Jun and ATF/CREB alters DNA binding specificity.
    Proc Natl Acad Sci U S A. 1991 May 1;88(9):3720-4 PMID: 1827203
  28. Two site-specific deletions and t(1;14) translocation restricted to human T-cell acute leukemias disrupt the 5' part of the tal-1 gene.
    Oncogene. 1991 Aug;6(8):1477-88 PMID: 1886719
  29. Jun inhibits myogenic differentiation.
    Oncogene. 1991 Oct;6(10):1759-66 PMID: 1656361
  30. v-jun oncogene prevents terminal differentiation and suppresses muscle-specific gene expression in ASV-17-infected muscle cells.
    Oncogene. 1991 Oct;6(10):1767-73 PMID: 1923502
  31. Purification, cloning, and RXR identity of the HeLa cell factor with which RAR or TR heterodimerizes to bind target sequences efficiently.
    Cell. 1992 Jan 24;68(2):377-95 PMID: 1310259
  32. Functional antagonism between c-Jun and MyoD proteins: a direct physical association.
    Cell. 1992 Feb 7;68(3):507-19 PMID: 1310896
  33. Modulation of normal erythroid differentiation by the endogenous thyroid hormone and retinoic acid receptors: a possible target for v-erbA oncogene action.
    Oncogene. 1992 Feb;7(2):217-27 PMID: 1347914
  34. c-jun and c-fos are expressed by human megakaryocytes.
    Exp Hematol. 1992 Aug;20(7):909-15 PMID: 1628710
  35. GATA and Ets cis-acting sequences mediate megakaryocyte-specific expression.
    Mol Cell Biol. 1993 Jan;13(1):668-76 PMID: 8417360
  36. A hepatitis B virus pre-S-retinoic acid receptor beta chimera transforms erythrocytic progenitor cells in vitro.
    Proc Natl Acad Sci U S A. 1993 Jan 1;90(1):89-93 PMID: 8093562
  37. A single point mutation in the v-ets oncogene affects both erythroid and myelomonocytic cell differentiation.
    Cell. 1988 Dec 23;55(6):1147-58 PMID: 3203383
  38. Cloning of cDNA for the major DNA-binding protein of the erythroid lineage through expression in mammalian cells.
    Nature. 1989 Jun 8;339(6224):446-51 PMID: 2725678
  39. Expression of the v-erbA product, an altered nuclear hormone receptor, is sufficient to transform erythrocytic cells in vitro.
    Cell. 1989 Jul 14;58(1):115-21 PMID: 2568887
  40. Leucine zippers of fos, jun and GCN4 dictate dimerization specificity and thereby control DNA binding.
    Nature. 1989 Aug 17;340(6234):568-71 PMID: 2505081
  41. Cis- and trans-acting elements involved in the regulation of the erythroid promoter of the human porphobilinogen deaminase gene.
    Proc Natl Acad Sci U S A. 1989 Sep;86(17):6548-52 PMID: 2771941
  42. Expression of c-jun protooncogene in human myelomonocytic cells.
    Blood. 1989 Oct;74(5):1811-6 PMID: 2477086
  43. The major human erythroid DNA-binding protein (GF-1): primary sequence and localization of the gene to the X chromosome.
    Proc Natl Acad Sci U S A. 1990 Jan;87(2):668-72 PMID: 2300555
  44. A new avian leukosis virus-based packaging cell line that uses two separate transcomplementing helper genomes.
    J Virol. 1990 Mar;64(3):1070-8 PMID: 2154593
  45. Role of the v-erbA and v-erbB oncogenes of avian erythroblastosis virus in erythroid cell transformation.
    Cell. 1983 Aug;34(1):7-9 PMID: 6309413
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.19.8837
Published
1993-10-01
Pages
8837-41
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC47455
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.90.19.8837 PMC Full Text View journal details
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