Home LiteratureArticle Details
PMID: 810603 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mason-Pfizer monkey virus: analysis and localization of virion proteins and glycoproteins.

Journal of virology ·Vol. 16 ·No. 5 ·1975-11-00 ·Pages 1208-19

Schochetman G, Kortright K, Schlom J

Abstract

The polypeptide composition of Mason-Pfizer monkey virus was determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Six major polypeptides of molecular weights 68,000, 27,000, 20,000, 14,000, 12,000, and 10,000 were resolved regardless of the cell type (i.e., two human and two rhesus) in which the virus was grown. Protein gp68 (68,000) represented the major virus glycoprotein and protein gp20 (20,000) represented a minor glycoprotein of the virion, again regardless of the cell type of origin of the virus. Protein gp68 appears to be located on the outer surface of the viral envelope, as demonstrated by lactoperoxidase catalyzed iodination of intact virions. Additional glycoproteins were shown to be virion associated; their presence depended, however, on the cell type in which the virus was propagated.

MeSH Terms
Animals Cell Line Glycoproteins/analysis Haplorhini Humans Macaca mulatta Molecular Weight Oncogenic Viruses/analysis,ultrastructure Peptides/analysis Viral Proteins/analysis
Chemicals
Glycoproteins Peptides Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schochetman G
Kortright K
Schlom J
References (34)
34 references, click to expand
  1. Evidence for crossing-over between avian tumor viruses based on analysis of viral RNAs.
    Proc Natl Acad Sci U S A. 1974 Oct;71(10):4254-8 PMID: 4372615
  2. The metabolism of glucosamine by tissue culture cells.
    Exp Cell Res. 1966 Mar;41(3):592-600 PMID: 4952045
  3. A new virus in a spontaneous mammary tumor of a rhesus monkey.
    Cancer Res. 1970 Aug;30(8):2081-6 PMID: 4195910
  4. A proposed nomenclature for the virion proteins of oncogenic RNA viruses.
    Virology. 1974 Aug;60(2):595-600 PMID: 4135911
  5. Morphological and biophysical properties of the Mason-Pfizer monkey virus.
    J Natl Cancer Inst. 1972 Feb;48(2):417-22 PMID: 4631464
  6. A study of the relationship of reticuloendotheliosis virus to the avian leukosis-sarcoma complex of viruses.
    Virology. 1973 Jun;53(2):287-99 PMID: 4351341
  7. Biochemical properties of oncornavirus polypeptides.
    Biochim Biophys Acta. 1974 Dec 31;355(3-4):220-35 PMID: 4376419
  8. Characterization and separation of viral DNA polymerase in mouse milk.
    Proc Natl Acad Sci U S A. 1973 Jul;70(7):2117-21 PMID: 4124683
  9. Purification, characterization, and comparison of the DNA polymerases from two primate RNA tumor viruses.
    J Virol. 1973 Sep;12(3):431-9 PMID: 4127027
  10. Immunological properties of two polypeptides of Mason-Pfizer monkey virus.
    J Virol. 1974 Jul;14(1):125-32 PMID: 4134467
  11. Oncornavirus-like particles in HeLa cells. II. Immunological characterization of the virus.
    Int J Cancer. 1974 Feb 15;13(2):254-61 PMID: 4131731
  12. Mason-Pfizer virus characterization: a similar virus in a human amniotic cell line.
    J Virol. 1973 Dec;12(6):1540-7 PMID: 4128382
  13. Serological analysis of reverse transcriptase of the Mason-Pfizer monkey virus.
    Virology. 1974 May;59(1):335-8 PMID: 4133137
  14. Isolation of oncornaviruses from continuous human cell cultures.
    Intervirology. 1973;1(1):19-26 PMID: 4129686
  15. Separation of B and C type virions by centrifugation in gentle density gradients.
    J Virol. 1974 May;13(5):1143-7 PMID: 4363254
  16. The nucleotide sequence complexity of avian tumor virus RNA.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3560-4 PMID: 4372625
  17. Cation preferences for poly(rC)-oligo(dG)-directed DNA synthesis by RNA tumor viruses and human milk particulates.
    Cancer Res. 1974 Dec;34(12):3509-15 PMID: 4371968
  18. Structure of the mouse mammary tumor virus: polypeptides and glycoproteins.
    J Virol. 1974 Feb;13(2):411-8 PMID: 4359300
  19. Structural characteristics of some murine RNA tumor viruses studied by lactoperoxidase iodination.
    Proc Natl Acad Sci U S A. 1973 Jan;70(1):36-40 PMID: 4346036
  20. Ultrastructural comparison of a virus from a Rhesus-monkey mammary carcinoma with four oncogenic RNA viruses.
    Proc Natl Acad Sci U S A. 1971 Jul;68(7):1603-7 PMID: 4327008
  21. RNA-dependent DNA polymerase activity in five RNA viruses: divalent cation requirements.
    Proc Natl Acad Sci U S A. 1970 Dec;67(4):1789-96 PMID: 4322157
  22. Avian tumor viruses.
    Adv Virus Res. 1965;11:293-385 PMID: 4285262
  23. Properties of mouse leukemia viruses. VII. The major viral glycoprotein of friend leukemia virus. Isolation and physicochemical properties.
    Virology. 1974 Sep;61(1):100-11 PMID: 4213320
  24. History of a rhesus monkey adenocarcinoma containing virus particles resembling oncogenic RNA viruses.
    J Natl Cancer Inst. 1972 May;48(5):1323-31 PMID: 4624149
  25. Interspecies antigenic determinants of the reverse transcriptases and p30 proteins of mammalian type C viruses.
    J Virol. 1975 Jun;15(6):1440-8 PMID: 49443
  26. Base sequence complexity of 35S avian myeloblastosis virus RNA determined by molecular hybridization kinetics.
    Cold Spring Harb Symp Quant Biol. 1975;39 Pt 2:869-74 PMID: 169041
  27. Detection of oncornavirus-like particles in HeLa cells. I. Fine structure and comparative morphological classification.
    Int J Cancer. 1974 Feb 15;13(2):246-53 PMID: 4817575
  28. Enzymatic iodination of Sindbis virus proteins.
    J Virol. 1973 May;11(5):730-5 PMID: 4736110
  29. The enzymatic iodination of the red cell membrane.
    J Cell Biol. 1972 Nov;55(2):390-405 PMID: 5076780
  30. Isolation and propagation of a virus from a spontaneous mammary carcinoma of a rhesus monkey.
    Cancer Res. 1970 Sep;30(9):2388-93 PMID: 4990901
  31. DNA polymerase activities and nucleic acid components of virions isolated from a spontaneous mammary carcinoma from a rhesus monkey.
    Proc Natl Acad Sci U S A. 1971 Jul;68(7):1613-7 PMID: 5283952
  32. RNA subunit structure of Mason-Pfizer monkey virus.
    J Virol. 1975 Feb;15(2):423-7 PMID: 803570
  33. Polypeptide composition of spleen necrosis virus, a reticuloendotheliosis virus.
    J Virol. 1975 May;15(5):1088-95 PMID: 1142473
  34. The polypeptides of influenza virus. V. Localization of polypeptides in the virion by iodination techniques.
    Virology. 1971 Dec;46(3):764-73 PMID: 5167658
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.16.5.1208-1219.1975
Published
1975-11-00
Pages
1208-19
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC355720
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.16.5.1208-1219.1975 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2