Abstract
Escherichia coli rnhA mutants lacking RNase HI chronically express the SOS response (T. Kogoma, X. Hong, G. W. Cadwell, K. G. Barnard, and T. Asai, Biochimie 75:89-99, 1993). Seventeen rpoB (Rifr) mutant alleles, which encode altered beta subunits of RNA polymerase, giving rise to resistance to rifampin, were screened for the ability to enhance or diminish constitutive expression of the SOS response in rnhA mutants. Two mutations, rpoB3595 and rpoB2, were found to enhance the SOS response 5- and 2.5-fold, respectively, only when RNase HI is absent. These mutations rendered rnhA mutant cells very sensitive to broth; i.e., the plating efficiency of the double mutants was drastically reduced when tested on broth plates. Two mutations, rpoB8 and rpoB3406, were found to diminish constitutive SOS expression in rnhA mutants by 43 and 30%, respectively. It was suggested that RNA polymerase may have a property that influences the size of DNA-RNA hybrids, the frequency of their formation, or both and that the property resides at least in part in the beta subunit of the polymerase.
MeSH Terms
Alleles
DNA-Directed RNA Polymerases/genetics,metabolism
Drug Resistance, Microbial/physiology
Escherichia coli/enzymology,genetics,growth & development
Gene Expression Regulation, Bacterial
Genes, Bacterial
Genotype
Macromolecular Substances
Recombinant Fusion Proteins/metabolism
Ribonuclease H/genetics,metabolism
Rifampin/toxicity
SOS Response, Genetics
Sequence Deletion
beta-Galactosidase/metabolism
Chemicals
Macromolecular Substances
Recombinant Fusion Proteins
DNA-Directed RNA Polymerases
Ribonuclease H
ribonuclease HI
beta-Galactosidase
Rifampin
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Kogoma T
Department of Cell Biology, University of New Mexico School of Medicine, Albuquerque 87131.
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