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PMID: 811643 Published · ppublish English Journal Article

Isolation of 30S and 50S active ribosomal subunits of Bacillus subtilis, Marburg strain.

Journal of bacteriology ·Vol. 124 ·No. 3 ·1975-12-00 ·Pages 1062-6

Guha S, Szulmajster J

Abstract

Active 30S and 50S ribosomal subunits were isolated from Bacillus subtilis. These subunits were able to perform not only protein synthesis in the presence of artificial or natural messenger ribonucleic acid but also the specific functions characteristic of each of the subunits. Thus the 30S subunits alone are able to bind formyl-methionyl-transfer ribonucleic acid, and the 50S subunits carry the peptidyl transferase activity.

MeSH Terms
Bacillus subtilis/analysis,enzymology,metabolism Bacterial Proteins/biosynthesis GTP Phosphohydrolases/metabolism Magnesium/pharmacology Peptide Biosynthesis Peptidyl Transferases/metabolism Phenylalanine/metabolism RNA, Bacterial/metabolism RNA, Messenger/metabolism RNA, Transfer/metabolism Ribosomes/enzymology,metabolism
Chemicals
Bacterial Proteins RNA, Bacterial RNA, Messenger Phenylalanine RNA, Transfer Peptidyl Transferases GTP Phosphohydrolases Magnesium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Guha S
Szulmajster J
References (16)
16 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.124.3.1062-1066.1975
Published
1975-12-00
Pages
1062-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC236006
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.124.3.1062-1066.1975 PMC Full Text View journal details
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