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PMID: 8123794 Published · ppublish English Comparative Study Journal Article

Cloning and characterization of a tomato GTPase-like gene related to yeast and Arabidopsis genes involved in vesicular transport.

Plant molecular biology ·Vol. 24 ·No. 3 ·1994-02-00 ·Pages 525-31

Davies C

Abstract

The deduced translation product of a tomato cDNA derived from a gene expressed in a number of tomato tissues of different developmental stages contained sequence motifs characteristic of the GTPase superfamily of proteins. The sequence was closely related to the Sar1 protein of Saccharomyces cerevisiae, a protein essential for the formation of protein transport vesicles at the endoplasmic reticulum (ER) (A. Nakano and M. Muramatsu, Cell Biol 109 (1989): 2677-2691). From analysis of the GTPase superfamily gene sequences, including the tomato SAR-like gene, it is proposed that the SAR genes comprise a distinct GTPase subfamily, presumably with a common, essential function in vesicular transport.

Related Genes
SAR
MeSH Terms
Amino Acid Sequence Arabidopsis/genetics Base Sequence Cloning, Molecular DNA, Complementary/metabolism GTP Phosphohydrolases/biosynthesis,genetics GTP-Binding Proteins/biosynthesis,genetics Gene Expression Genes, Plant Molecular Sequence Data Monomeric GTP-Binding Proteins Phylogeny Protein Biosynthesis Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Vegetables/enzymology,genetics,growth & development Vesicular Transport Proteins
Chemicals
DNA, Complementary SAR protein, Lycopersicon esculentum Saccharomyces cerevisiae Proteins Vesicular Transport Proteins GTP Phosphohydrolases GTP-Binding Proteins Monomeric GTP-Binding Proteins SAR1 protein, S cerevisiae
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Davies C
CSIRO, Division of Horticulture, North Ryde, NSW, Australia.
References (36)
36 references, click to expand
  1. Isolation and analysis of cDNAs encoding small GTP-binding proteins of Arabidopsis thaliana.
    Gene. 1991 Dec 15;108(2):259-64 PMID: 1748311
  2. Distinct sets of SEC genes govern transport vesicle formation and fusion early in the secretory pathway.
    Cell. 1990 May 18;61(4):723-33 PMID: 2188733
  3. Human ADP-ribosylation factors. A functionally conserved family of GTP-binding proteins.
    J Biol Chem. 1991 Feb 5;266(4):2606-14 PMID: 1899243
  4. A membrane glycoprotein, Sec12p, required for protein transport from the endoplasmic reticulum to the Golgi apparatus in yeast.
    J Cell Biol. 1988 Sep;107(3):851-63 PMID: 3047151
  5. Molecular dissection of the secretory pathway.
    Nature. 1992 Jan 30;355(6359):409-15 PMID: 1734280
  6. Ovalbumin messenger ribonucleic acid translation. Comparable rates of polypeptide initiation and elongation on ovalbumin and globin messenger ribonucleic acid in a rabbit reticulocyte lysate.
    J Biol Chem. 1973 Mar 25;248(6):2095-106 PMID: 4690593
  7. The murine rac1 gene: cDNA cloning, tissue distribution and regulated expression of rac1 mRNA by disassembly of actin microfilaments.
    Oncogene. 1991 May;6(5):863-6 PMID: 1905006
  8. A novel GTP-binding protein, Sar1p, is involved in transport from the endoplasmic reticulum to the Golgi apparatus.
    J Cell Biol. 1989 Dec;109(6 Pt 1):2677-91 PMID: 2512296
  9. Vesicle-mediated protein sorting.
    Annu Rev Biochem. 1992;61:471-516 PMID: 1497318
  10. Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products.
    Nucleic Acids Res. 1991 Mar 11;19(5):1154 PMID: 2020552
  11. ADP-ribosylation factor is functionally and physically associated with the Golgi complex.
    Proc Natl Acad Sci U S A. 1990 Feb;87(3):1238-42 PMID: 2105501
  12. Coding sequences of human ralA and ralB cDNAs.
    Nucleic Acids Res. 1989 Jun 12;17(11):4380 PMID: 2662142
  13. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  14. Sequences of the bovine and yeast ADP-ribosylation factor and comparison to other GTP-binding proteins.
    Proc Natl Acad Sci U S A. 1988 Jul;85(13):4620-4 PMID: 3133654
  15. The GTP-binding Sar1 protein is localized to the early compartment of the yeast secretory pathway.
    Biochim Biophys Acta. 1991 Jul 10;1093(2-3):135-43 PMID: 1907491
  16. Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologue.
    Nature. 1983 Mar 3;302(5903):33-7 PMID: 6298635
  17. Genes in S. cerevisiae encoding proteins with domains homologous to the mammalian ras proteins.
    Cell. 1984 Mar;36(3):607-12 PMID: 6365329
  18. Molecular characterization of CDC42, a Saccharomyces cerevisiae gene involved in the development of cell polarity.
    J Cell Biol. 1990 Jul;111(1):143-52 PMID: 2164028
  19. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  20. cDNA cloning and expression of an Arabidopsis GTP-binding protein of the ARF family.
    FEBS Lett. 1993 Jan 25;316(2):133-6 PMID: 8420798
  21. The GTPase superfamily: conserved structure and molecular mechanism.
    Nature. 1991 Jan 10;349(6305):117-27 PMID: 1898771
  22. Molecular characterization of tobacco cDNAs encoding two small GTP-binding proteins.
    Plant Mol Biol. 1992 Aug;19(5):847-57 PMID: 1643285
  23. Characterization of four novel ras-like genes expressed in a human teratocarcinoma cell line.
    Mol Cell Biol. 1990 Apr;10(4):1793-8 PMID: 2108320
  24. Cloning and characterization of the gene encoding the ADP-ribosylation factor in Candida albicans.
    Gene. 1992 Jan 2;110(1):123-8 PMID: 1544569
  25. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  26. Premature initiation of mitosis in yeast lacking RCC1 or an interacting GTPase.
    Cell. 1991 Jul 26;66(2):347-60 PMID: 1855255
  27. A novel ras-related rgp1 gene encoding a GTP-binding protein has reduced expression in 5-azacytidine-induced dwarf rice.
    Mol Gen Genet. 1991 Aug;228(1-2):227-32 PMID: 1715972
  28. Characterization of two members of the rho gene family from the yeast Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1987 Feb;84(3):779-83 PMID: 3543936
  29. Chemical and immunological characterization of the 21-kDa ADP-ribosylation factor of adenylate cyclase.
    J Biol Chem. 1988 Jun 15;263(17):8282-7 PMID: 3131341
  30. A yeast gene encoding a protein homologous to the human c-has/bas proto-oncogene product.
    Nature. 1983 Dec 15-21;306(5944):704-7 PMID: 6318115
  31. CLUSTAL V: improved software for multiple sequence alignment.
    Comput Appl Biosci. 1992 Apr;8(2):189-91 PMID: 1591615
  32. Sec12p-dependent membrane binding of the small GTP-binding protein Sar1p promotes formation of transport vesicles from the ER.
    J Cell Biol. 1991 Aug;114(4):663-70 PMID: 1907973
  33. The ras superfamily of GTP-binding proteins: guidelines on nomenclature.
    FASEB J. 1992 May;6(8):2512-3 PMID: 1592203
  34. Reconstitution of GTP-binding Sar1 protein function in ER to Golgi transport.
    J Cell Biol. 1991 Aug;114(4):671-9 PMID: 1907974
  35. Nucleotide sequence of human rho cDNA clone 12.
    Nucleic Acids Res. 1987 Feb 25;15(4):1869 PMID: 3822842
  36. Fission yeast and a plant have functional homologues of the Sar1 and Sec12 proteins involved in ER to Golgi traffic in budding yeast.
    EMBO J. 1992 Nov;11(11):4205-11 PMID: 1396601
Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00024120
Published
1994-02-00
Pages
525-31
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
L12051
External Links
PubMed source DOI: 10.1007/BF00024120 View journal details
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