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PMID: 8132742 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Leukotriene A4 hydrolase in human bronchoalveolar lavage fluid.

The Journal of clinical investigation ·Vol. 93 ·No. 3 ·1994-03-00 ·Pages 1042-50

Munafo DA, Shindo K, Baker JR, Bigby TD

Abstract

We examined cell-free human bronchoalveolar lavage fluid (BALF) for enzymes of the 5-lipoxygenase pathway. BALF was obtained from six patients who were active smokers and six nonsmokers. Enzymatic activity in cell-free BALF was assessed by specific assays for leukotriene (LT) A4 hydrolase, 5-lipoxygenase, and LTC4 synthase using HPLC. Only LTA4 hydrolase enzymatic activity was found. This activity ranged from 101 to 667 when expressed as picomoles of LTB4 produced per milliliter BALF. Enzymatic activity in smokers vs nonsmokers was 484 +/- 120 vs 129 +/- 32 pmol LTB4/ml BALF (mean +/- SD, P < 0.0001). There were no leukotrienes found in BALF before assay. Immunoblot analysis revealed an immunoreactive band at a relative molecular mass of 69,000 D in all samples, consistent with LTA4 hydrolase, but no evidence of 5-lipoxygenase. BALF had greater LTA4 hydrolase activity per milligram of protein than neutrophil cytosol, epithelial cell cytosol, plasma, or serum. The synthesis of LTB4 was significantly increased when neutrophils were stimulated in BALF. These data indicate the selective presence of LTA4 hydrolase in BALF which is significantly increased in smokers. This enzyme in BALF may contribute to the inflammatory response in tobacco-related lung disease.

MeSH Terms
Adult Aged Aminopeptidases/metabolism Arachidonate 5-Lipoxygenase/metabolism Bronchoalveolar Lavage Fluid/chemistry Epoxide Hydrolases/metabolism Female Glutathione Transferase/metabolism Humans Immunoblotting Male Middle Aged Smoking/metabolism
Chemicals
Arachidonate 5-Lipoxygenase Glutathione Transferase Epoxide Hydrolases Aminopeptidases leukotriene-C4 synthase leukotriene A4 hydrolase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Munafo D A
Department of Medicine, University of California, San Diego.
Shindo K
Baker J R
Bigby T D
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40 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI117053
Published
1994-03-00
Pages
1042-50
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC294032
Subset
IM
Grants
NHLBI NIH HHS · HL-01888 · United States
External Links
PubMed source DOI: 10.1172/JCI117053 PMC Full Text View journal details
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