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PMID: 8135524 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Recognition of chimeric small-subunit ribosomal DNAs composed of genes from uncultivated microorganisms.

Applied and environmental microbiology ·Vol. 60 ·No. 2 ·1994-02-00 ·Pages 746-8

Kopczynski ED, Bateson MM, Ward DM

Abstract

When PCR was used to recover small-subunit (SSU) rRNA genes from a hot spring cyanobacterial mat community, chimeric SSU rRNA sequences which exhibited little or no secondary structural abnormality were recovered. They were revealed as chimeras of SSU rRNA genes of uncultivated species through separate phylogenetic analysis of short sequence domains.

Keywords
NASA Discipline Exobiology Non-NASA Center
MeSH Terms
Base Sequence Chimera/genetics Clone Cells Cyanobacteria/genetics DNA, Fungal/genetics,isolation & purification DNA, Ribosomal/genetics,isolation & purification Molecular Sequence Data
Chemicals
DNA, Fungal DNA, Ribosomal
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kopczynski E D
Department of Microbiology, Montana State University, Bozeman 59717.
Bateson M M
Ward D M
Investigators
1 investigators, click to expand
Ward D M
MT St U, Bozeman
References (14)
14 references, click to expand
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.60.2.746-748.1994
Published
1994-02-00
Pages
746-8
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC201378
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.60.2.746-748.1994 PMC Full Text View journal details
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