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PMID: 8143801 Published · ppublish English Comparative Study Journal Article Review

Gene conversion in the chicken immunoglobulin locus: a paradigm of homologous recombination in higher eukaryotes.

Experientia ·Vol. 50 ·No. 3 ·1994-03-15 ·Pages 270-6

Bezzubova OY, Buerstedde JM

Abstract

Gene conversion was first defined in yeast as a type of homologous recombination in which the donor sequence does not change. In chicken B cells, gene conversion builds the antigen receptor repertoire by introducing sequence diversity into the immunoglobulin genes. Immunoglobulin gene conversion continues at high frequency in an avian leukosis virus induced chicken B cell line. This cell line can be modified by homologous integration of transfected DNA constructs offering a model system for studying gene conversion in higher eukaryotes. In search for genes which might participate in chicken immunoglobulin gene conversion, we have identified chicken counterparts of the yeast RAD51, RAD52, and RAD54 genes. Disruption and overexpression of these genes in the chicken B cell line may clarify their role in gene conversion and gene targeting.

MeSH Terms
Amino Acid Sequence Animals Antibody Diversity Avian Proteins B-Lymphocytes/physiology Chickens/genetics DNA Helicases DNA Repair DNA Repair Enzymes DNA-Binding Proteins/physiology Epistasis, Genetic Fungal Proteins/physiology Gene Conversion Genes, Immunoglobulin Immunoglobulin Light Chains/genetics Mice Molecular Sequence Data Proteins/physiology Rad51 Recombinase Rad52 DNA Repair and Recombination Protein Recombination, Genetic Saccharomyces cerevisiae Proteins Sequence Alignment Sequence Homology, Amino Acid
Chemicals
Avian Proteins DNA-Binding Proteins Fungal Proteins Immunoglobulin Light Chains Proteins Rad52 DNA Repair and Recombination Protein Rag2 protein, mouse Saccharomyces cerevisiae Proteins V(D)J recombination activating protein 2 RAD51 protein, Gallus gallus Rad51 Recombinase Rad51 protein, mouse RAD54 protein, S cerevisiae DNA Helicases DNA Repair Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bezzubova O Y
Basel Institute for Immunology, Switzerland.
Buerstedde J M
References (56)
56 references, click to expand
  1. Creation of immunoglobulin diversity by intrachromosomal gene conversion.
    Trends Genet. 1992 Dec;8(12):416-22 PMID: 1492367
  2. Repair of interstrand cross-links in DNA of Saccharomyces cerevisiae requires two systems for DNA repair: the RAD3 system and the RAD51 system.
    Mol Gen Genet. 1981;182(2):196-205 PMID: 7026973
  3. The V(D)J recombination activating gene, RAG-1.
    Cell. 1989 Dec 22;59(6):1035-48 PMID: 2598259
  4. Induction of dominant lethality by x-rays in radiosensitive strain of yeast.
    Mutat Res. 1973 Oct;20(1):45-51 PMID: 4586554
  5. Molecular analysis of V(D)J recombination.
    Annu Rev Genet. 1992;26:425-46 PMID: 1482120
  6. Light chain gene conversion continues at high rate in an ALV-induced cell line.
    EMBO J. 1990 Mar;9(3):921-7 PMID: 2155784
  7. Rad52-independent mitotic gene conversion in Saccharomyces cerevisiae frequently results in chromosomal loss.
    Genetics. 1985 Sep;111(1):7-22 PMID: 3896928
  8. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.
    Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5547-51 PMID: 1319065
  9. A genetic study of x-ray sensitive mutants in yeast.
    Mutat Res. 1974 Sep;24(3):281-92 PMID: 4606119
  10. Avian B-cell development: generation of an immunoglobulin repertoire by gene conversion.
    Annu Rev Immunol. 1991;9:219-41 PMID: 1910677
  11. Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein.
    Cell. 1992 May 1;69(3):457-70 PMID: 1581961
  12. Somatic mutation gains its place among the generators of diversity.
    Cell. 1981 Nov;26(3 Pt 1):295-6 PMID: 7326743
  13. V(D)J recombination in mouse thymocytes: double-strand breaks near T cell receptor delta rearrangement signals.
    Cell. 1992 Apr 3;69(1):41-53 PMID: 1313336
  14. Similarity of the yeast RAD51 filament to the bacterial RecA filament.
    Science. 1993 Mar 26;259(5103):1896-9 PMID: 8456314
  15. Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA.
    Nat Genet. 1993 Jul;4(3):239-43 PMID: 8358431
  16. The defect in murine severe combined immune deficiency: joining of signal sequences but not coding segments in V(D)J recombination.
    Cell. 1988 Oct 7;55(1):7-16 PMID: 3167977
  17. Nucleotide excision repair I: from E. coli to yeast.
    Trends Genet. 1993 May;9(5):173-7 PMID: 8337754
  18. ERCC6, a member of a subfamily of putative helicases, is involved in Cockayne's syndrome and preferential repair of active genes.
    Cell. 1992 Dec 11;71(6):939-53 PMID: 1339317
  19. Templated insertions in the rearranged chicken IgL V gene segment arise by intrachromosomal gene conversion.
    Genes Dev. 1990 Apr;4(4):536-47 PMID: 2113878
  20. Targeted homologous recombination at the endogenous adenine phosphoribosyltransferase locus in Chinese hamster cells.
    Proc Natl Acad Sci U S A. 1989 Jun;86(12):4574-8 PMID: 2734308
  21. scid mutation in mice confers hypersensitivity to ionizing radiation and a deficiency in DNA double-strand break repair.
    Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1394-7 PMID: 1996340
  22. Somatic generation of antibody diversity.
    Nature. 1983 Apr 14;302(5909):575-81 PMID: 6300689
  23. Impairment of V(D)J recombination in double-strand break repair mutants.
    Science. 1993 Apr 9;260(5105):207-10 PMID: 8469973
  24. Chicken IgL gene rearrangement involves deletion of a circular episome and addition of single nonrandom nucleotides to both coding segments.
    Cell. 1989 Mar 10;56(5):785-91 PMID: 2493991
  25. The scid mutation in mice causes a general defect in DNA repair.
    Nature. 1990 Oct 4;347(6292):479-82 PMID: 2215662
  26. Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes.
    Nucleic Acids Res. 1989 Jun 26;17 (12 ):4713-30 PMID: 2546125
  27. A single rearrangement event generates most of the chicken immunoglobulin light chain diversity.
    Cell. 1985 Feb;40(2):283-91 PMID: 3917859
  28. The fission yeast rad22 gene, having a function in mating-type switching and repair of DNA damages, encodes a protein homolog to Rad52 of Saccharomyces cerevisiae.
    Nucleic Acids Res. 1993 Dec 25;21(25):5940-4 PMID: 8290356
  29. A chicken RAD51 homologue is expressed at high levels in lymphoid and reproductive organs.
    Nucleic Acids Res. 1993 Apr 11;21(7):1577-80 PMID: 8479908
  30. Chicken IgL variable region gene conversions display pseudogene donor preference and 5' to 3' polarity.
    Genes Dev. 1990 Apr;4(4):548-58 PMID: 2113879
  31. Increased ratio of targeted to random integration after transfection of chicken B cell lines.
    Cell. 1991 Oct 4;67(1):179-88 PMID: 1913816
  32. Selection for B cells with productive IgL gene rearrangements occurs in the bursa of Fabricius during chicken embryonic development.
    Genes Dev. 1989 Jun;3(6):838-47 PMID: 2501152
  33. Somatic diversification of immunoglobulin heavy chain VDJ genes: evidence for somatic gene conversion in rabbits.
    Cell. 1990 Nov 30;63(5):987-97 PMID: 2124176
  34. The RAD52 gene is required for homothallic interconversion of mating types and spontaneous mitotic recombination in yeast.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):503-7 PMID: 6987653
  35. Somatic diversification of the chicken immunoglobulin light chain gene is limited to the rearranged variable gene segment.
    Cell. 1987 Feb 13;48(3):369-78 PMID: 3100049
  36. A link between double-strand break-related repair and V(D)J recombination: the scid mutation.
    Proc Natl Acad Sci U S A. 1991 May 15;88(10):4061-5 PMID: 1709732
  37. Identification of a chicken RAD52 homologue suggests conservation of the RAD52 recombination pathway throughout the evolution of higher eukaryotes.
    Nucleic Acids Res. 1993 Dec 25;21(25):5945-9 PMID: 8290357
  38. DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression.
    Cell. 1992 May 1;69(3):439-56 PMID: 1581960
  39. A severe combined immunodeficiency mutation in the mouse.
    Nature. 1983 Feb 10;301(5900):527-30 PMID: 6823332
  40. Primary structure of the RAD52 gene in Saccharomyces cerevisiae.
    Mol Cell Biol. 1984 Dec;4(12):2735-44 PMID: 6098821
  41. Insertion of DNA sequences into the human chromosomal beta-globin locus by homologous recombination.
    Nature. 1985 Sep 19-25;317(6034):230-4 PMID: 2995814
  42. RAG-2 expression is not essential for chicken immunoglobulin gene conversion.
    Proc Natl Acad Sci U S A. 1992 May 1;89(9):4023-7 PMID: 1570327
  43. Restricted VH gene usage and generation of antibody diversity in rabbit.
    Annu Rev Immunol. 1992;10:593-616 PMID: 1590997
  44. Semidominant suppressors of Srs2 helicase mutations of Saccharomyces cerevisiae map in the RAD51 gene, whose sequence predicts a protein with similarities to procaryotic RecA proteins.
    Mol Cell Biol. 1992 Jul;12(7):3224-34 PMID: 1620127
  45. High frequency targeting of genes to specific sites in the mammalian genome.
    Cell. 1986 Feb 14;44(3):419-28 PMID: 3002636
  46. The effect of alterations in myc gene expression on B cell development in the bursa of Fabricius.
    Cell. 1987 Nov 6;51(3):371-81 PMID: 3499231
  47. Selective expression of RAG-2 in chicken B cells undergoing immunoglobulin gene conversion.
    Cell. 1991 Jan 11;64(1):201-8 PMID: 1986866
  48. Sequence of RAD54, a Saccharomyces cerevisiae gene involved in recombination and repair.
    Gene. 1991 Jul 31;104(1):103-6 PMID: 1916269
  49. Repair of double-strand breaks in a temperature conditional radiation-sensitive mutant of Saccharomyces cerevisiae.
    Mutat Res. 1982 Jan;103(1):19-24 PMID: 7035912
  50. Nucleotide excision repair. II: From yeast to mammals.
    Trends Genet. 1993 Jun;9(6):211-7 PMID: 8337762
  51. RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination.
    Science. 1990 Jun 22;248(4962):1517-23 PMID: 2360047
  52. Mating-type gene switching in Saccharomyces cerevisiae.
    Trends Genet. 1992 Dec;8(12 ):446-52 PMID: 1492369
  53. Rearrangement of chicken immunoglobulin genes is not an ongoing process in the embryonic bursa of Fabricius.
    Proc Natl Acad Sci U S A. 1986 May;83(10):3336-40 PMID: 3010290
  54. A hyperconversion mechanism generates the chicken light chain preimmune repertoire.
    Cell. 1987 Feb 13;48(3):379-88 PMID: 3100050
  55. The chicken B cell compartment.
    Science. 1987 Nov 20;238(4830):1094-8 PMID: 3317827
  56. The repair of double-strand breaks in the nuclear DNA of Saccharomyces cerevisiae and its genetic control.
    Mol Gen Genet. 1976 Jan 16;143(2):119-29 PMID: 765749
Article Info
Journal
Experientia
Abbr.
Experientia
ISSN
0014-4754
DOI
10.1007/BF01924010
Published
1994-03-15
Pages
270-6
Language
English
Region
Switzerland
NLM ID
0376547
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01924010
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