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PMID: 8290357 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of a chicken RAD52 homologue suggests conservation of the RAD52 recombination pathway throughout the evolution of higher eukaryotes.

Nucleic acids research ·Vol. 21 ·No. 25 ·1993-12-25 ·Pages 5945-9

Bezzubova OY, Schmidt H, Ostermann K, Heyer WD, Buerstedde JM

Abstract

Degenerate oligonucleotides encoding conserved regions of the Rad52 protein of S. cerevisiae and its homologue, the Rad22 protein of S. pombe, were used to clone a chicken RAD52 counterpart by the polymerase chain reaction. Sequence comparison of the chicken and yeast proteins reveals a strongly conserved region between positions 40 and 178 of the chicken Rad52 sequence indicating that this part of the protein is under strong evolutionary pressure. The first 39 amino acids and the 3' end of the chicken Rad52 homologue does not share significant similarity with the yeast proteins. High abundance of the mRNA in testis makes it likely that the chicken Rad52 protein plays a role in meiotic recombination.

Related Genes
RAD22 RAD52
MeSH Terms
Amino Acid Sequence Animals Base Sequence Biological Evolution Chickens Cloning, Molecular Conserved Sequence DNA DNA-Binding Proteins/genetics Fungal Proteins/genetics Molecular Sequence Data Polymerase Chain Reaction Rad52 DNA Repair and Recombination Protein Recombination, Genetic Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid
Chemicals
DNA-Binding Proteins Fungal Proteins RAD52 protein, S cerevisiae Rad52 DNA Repair and Recombination Protein Saccharomyces cerevisiae Proteins DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bezzubova O Y
Basel Institut of Immunology, Switzerland.
Schmidt H
Ostermann K
Heyer W D
Buerstedde J M
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32 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.25.5945
Published
1993-12-25
Pages
5945-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC310479
Subset
IM
Databases
GENBANK
U01047
External Links
PubMed source DOI: 10.1093/nar/21.25.5945 PMC Full Text View journal details
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