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PMID: 8144488 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Review

TonB protein and energy transduction between membranes.

Journal of bioenergetics and biomembranes ·Vol. 25 ·No. 6 ·1993-12-00 ·Pages 591-601

Postle K

Abstract

TonB protein couples cytoplasmic membrane electrochemical potential to active transport of iron-siderophore complexes and vitamin B12 through high-affinity outer membrane receptors of Gram-negative bacteria. The mechanism of energy transduction remains to be determined, but important concepts have already begun to emerge. Consistent with its function, TonB is anchored in the cytoplasmic membrane by its uncleaved amino terminus while largely occupying the periplasm. Both the connection to the cytoplasmic membrane and the amino acid sequences of the anchor are essential for activity. TonB directly associates with a number of envelope proteins, among them the outer membrane receptors and cytoplasmic membrane protein ExbB. ExbB and TonB interact through their respective transmembrane domains. ExbB is proposed to recycle TonB to an active conformation following energy transduction to the outer membrane. TonB most likely associates with the outer membrane receptors through its carboxy terminus, which is required for function. In contrast, the novel proline-rich region of TonB can be deleted without affecting function. A model that incorporates this information, as well as tempered speculation, is presented.

Related Genes
tonB
MeSH Terms
Amino Acid Sequence Bacteria/genetics,metabolism Bacterial Outer Membrane Proteins/chemistry,metabolism Bacterial Proteins/chemistry,genetics,metabolism Cell Membrane/chemistry,metabolism Escherichia coli/genetics,metabolism Escherichia coli Proteins Membrane Potentials Membrane Proteins/chemistry,genetics,metabolism Molecular Sequence Data Sequence Homology, Amino Acid
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins Escherichia coli Proteins Membrane Proteins tonB protein, Bacteria tonB protein, E coli
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Postle K
Department of Microbiology, Washington State University, Pullman 99164-4233.
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Article Info
Journal
Journal of bioenergetics and biomembranes
Abbr.
J Bioenerg Biomembr
ISSN
0145-479X
DOI
10.1007/BF00770246
Published
1993-12-00
Pages
591-601
Language
English
Region
United States
NLM ID
7701859
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00770246 View journal details
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