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PMID: 8146146 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

ocs element promoter sequences are activated by auxin and salicylic acid in Arabidopsis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 7 ·1994-03-29 ·Pages 2507-11

Zhang B, Singh KB

Abstract

ocs elements are a group of promoter elements that have been exploited by two distinct groups of plant pathogens, Agrobacterium and certain viruses, to express genes in plants. We examined the activity of single and multiple ocs elements linked to a minimal plant promoter and the uidA reporter gene in transgenic Arabidopsis. beta-Glucuronidase activity was detected only in root tips and in callus tissue after auxin treatment. A more sensitive assay revealed that auxin treatment also increased ocs element activity in aerial parts of the plant, although the absolute levels of ocs element activity were greater in roots. The response of ocs elements to exogenous auxin began within 1 h. Salicylic acid, a disease-resistance signal in plants, also increased ocs element activity in both roots and aerial parts of the plant. The question of whether the induction in ocs element activity is mediated through auxin and/or salicylic acid signal transduction pathways or is part of a more general stress response is discussed.

Related Genes
uidA
MeSH Terms
Arabidopsis/genetics Base Sequence Biological Transport Gene Expression Regulation/drug effects Genes, Plant/genetics Genes, Reporter Glucuronidase/biosynthesis Herbicides/pharmacology Indoleacetic Acids/pharmacology Molecular Sequence Data Phthalimides/pharmacology Promoter Regions, Genetic/genetics Salicylates/pharmacology Salicylic Acid
Chemicals
Herbicides Indoleacetic Acids Phthalimides Salicylates alpha-naphthylphthalamic acid Glucuronidase Salicylic Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zhang B
Department of Biology, University of California, Los Angeles 90024.
Singh K B
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39 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.7.2507
Published
1994-03-29
Pages
2507-11
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC43398
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.91.7.2507 PMC Full Text View journal details
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