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PMID: 8151779 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Simian virus 40 small-t antigen stimulates viral DNA replication in permissive monkey cells.

Journal of virology ·Vol. 68 ·No. 5 ·1994-05-00 ·Pages 3138-44

Cicala C, Avantaggiati ML, Graessmann A, Rundell K, Levine AS, Carbone M

Abstract

The simian virus 40 (SV40) large-T antigen is essential for SV40 DNA replication and for late viral gene expression, but the role of the SV40 small-t antigen in these processes is still unclear. We have previously demonstrated that small t inhibits SV40 DNA replication in vitro. In this study, we investigated the effect of small t on SV40 replication in cultured cells. CV1 monkey cell infection experiments indicated that mutant viruses that lack small t replicate less efficiently than the wild-type virus. We next microinjected CV1 cells with SV40 DNA with and without purified small-t protein and analyzed viral DNA replication efficiency by Southern blotting. Replication of either wild-type SV40 or small-t deletion mutant DNA was increased three- to fivefold in cells coinjected with purified small t. Thus, in contrast to our in vitro observation, small t stimulated viral DNA replication in vivo. This result suggests that small t has cellular effects that are not detectable in a reconstituted in vitro replication system. We also found that small t stimulated progression of permissive monkey cells--but not of nonpermissive rodent cells--from G0-G1 to the S phase of the cell cycle, possibly leading to an optimal intracellular environment for viral replication.

MeSH Terms
Animals Antigens, Viral, Tumor/genetics,metabolism,pharmacology Cell Cycle/drug effects Cells, Cultured Chlorocebus aethiops DNA Replication/drug effects DNA, Viral/biosynthesis Mice Microinjections Mutation Phosphorylation Protein Kinases/metabolism Simian virus 40/growth & development,immunology Virus Replication
Chemicals
Antigens, Viral, Tumor DNA, Viral Protein Kinases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Cicala C
Section on DNA Replication, Repair, and Mutagenesis, National Institute of Child Health and Human Development, Bethesda, Maryland 20892.
Avantaggiati M L
Graessmann A
Rundell K
Levine A S
Carbone M
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.68.5.3138-3144.1994
Published
1994-05-00
Pages
3138-44
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC236804
Subset
IM
Grants
NCI NIH HHS · CA-21327 · United States
External Links
PubMed source DOI: 10.1128/JVI.68.5.3138-3144.1994 PMC Full Text View journal details
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