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PMID: 8159735 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Evolution of translational elongation factor (EF) sequences: reliability of global phylogenies inferred from EF-1 alpha(Tu) and EF-2(G) proteins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 8 ·1994-04-12 ·Pages 3255-9

Creti R, Ceccarelli E, Bocchetta M, Sanangelantoni AM, Tiboni O, Palm P, Cammarano P

Abstract

The EF-2 coding genes of the Archaea Pyrococcus woesei and Desulfurococcus mobilis were cloned and sequenced. Global phylogenies were inferred by alternative tree-making methods from available EF-2(G) sequence data and contrasted with phylogenies constructed from the more conserved but shorter EF-1 alpha(Tu) sequences. Both the monophyly (sensu Hennig) of Archaea and their subdivision into the kingdoms Crenarchaeota and Euryarchaeota are consistently inferred by analysis of EF-2(G) sequences, usually at a high bootstrap confidence level. In contrast, EF-1 alpha(Tu) phylogenies tend to be inconsistent with one another and show low bootstrap confidence levels. While evolutionary distance and DNA maximum parsimony analyses of EF-1 alpha(Tu) sequences do show archaeal monophyly, protein parsimony and DNA maximum-likelihood analyses of these data do not. In no case, however, do any of the tree topologies inferred from EF-1 alpha(Tu) sequence analyses receive significant bootstrap support.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Likelihood Functions Molecular Sequence Data Peptide Elongation Factor 1 Peptide Elongation Factor G Peptide Elongation Factors/genetics Phylogeny Sequence Alignment Sequence Homology, Amino Acid
Chemicals
Bacterial Proteins Peptide Elongation Factor 1 Peptide Elongation Factor G Peptide Elongation Factors
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Creti R
Istituto Pasteur-Fondazione Cenci-Bolognetti, Dipt. Biopatologia Umana, Università di Roma I, Policlinico Umberto I., Italy.
Ceccarelli E
Bocchetta M
Sanangelantoni A M
Tiboni O
Palm P
Cammarano P
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44 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.8.3255
Published
1994-04-12
Pages
3255-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC43555
Subset
IM
Databases
GENBANK
X68014
External Links
PubMed source DOI: 10.1073/pnas.91.8.3255 PMC Full Text View journal details
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