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PMID: 8162323 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Chromosomal locations of four minor rDNA loci and a marker microsatellite sequence in barley.

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology ·Vol. 2 ·No. 1 ·1994-01-00 ·Pages 65-71

Pedersen C, Linde-Laursen I

Abstract

Four minor rDNA loci have been mapped physically to barley (Hordeum vulgare L.) chromosomes 1 (7l), 2 (2l), 4 (4l), and 5 (1l) by a two-step in situ hybridization procedure including a GAA microsatellite sequence. Reprobing with the microsatellite resulted in a distinct banding pattern, resembling the C-banding pattern, which enabled unequivocal chromosome identification. This study suggests that gene mapping accuracy may be improved by using probes with well-characterized and narrow hybridization sites as cytological markers which are situated close to the gene locus. One of the rDNA loci is located about 54% out on the short arm of chromosome 4 and it has not previously been reported in barley. We have designated the new locus Nor-l6. rDNA loci on homoeologous group 4 chromosomes have not yet been reported in other Triticeae species. The origin of these 4 minor rDNA loci is discussed in relation to their equilocal distribution on the chromosomes.

MeSH Terms

Chromosome Mapping/methods DNA, Ribosomal/genetics DNA, Satellite/genetics Genetic Markers Hordeum/ultrastructure In Situ Hybridization, Fluorescence RNA, Ribosomal/genetics RNA, Ribosomal, 18S/genetics Repetitive Sequences, Nucleic Acid

Chemicals

DNA, Ribosomal DNA, Satellite Genetic Markers RNA, Ribosomal RNA, Ribosomal, 18S RNA, ribosomal, 26S

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Pedersen C

Environmental Science and Technology Department, Risø National Laboratory, Roskilde, Denmark.

Linde-Laursen I

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Article Info

Journal

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology

Abbr.

Chromosome Res

ISSN

0967-3849

DOI

10.1007/BF01539456

Published

1994-01-00

Pages

65-71

Language

English

Region

Netherlands

NLM ID

9313452

Subset

External Links

PubMed source DOI: 10.1007/BF01539456 View journal details

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Chromosomal locations of four minor rDNA loci and a marker microsatellite sequence in barley.

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