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PMID: 8165137 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structure and regulation of the chicken erythroid delta-aminolevulinate synthase gene.

Nucleic acids research ·Vol. 22 ·No. 7 ·1994-04-11 ·Pages 1226-33

Lim KC, Ishihara H, Riddle RD, Yang Z, Andrews N, Yamamoto M, Engel JD

Abstract

Erythroid cells regulate heme biosynthesis in a manner that is distinct from all other cell types. While heme negatively regulates the synthesis of the housekeeping delta-aminolevulinate synthase (ALAS-N) in all non-erythroid cells, the expression of an erythroid-specific isozyme (ALAS-E) is developmentally regulated in red blood cells. As a first step towards understanding the molecular basis for the transcriptional regulation of ALAS-E during erythropoiesis, we cloned and characterized the chicken ALAS-E locus. This gene spans 18 kbp and is composed of eleven exons. The intron/exon structure of erythroid ALAS was found to be conserved among several vertebrate species. Direct RNA sequencing identified a 5' untranslated region that is derived from two continuous exons and is predicted to form a very stable stem-loop structure that bears resemblance to the ferritin iron-responsive element. Tissue-specific expression of the ALAS-E gene was analyzed by transient transfection assays in hematopoietic cells of both erythroid and non-erythroid origins. These experiments identified distal (-784 to -505 bp) and proximal (-155 to +21 bp) promoter elements which are required for high level, erythroid-specific transcription.

Related Genes
ALAS-E
MeSH Terms
5-Aminolevulinate Synthetase/genetics Animals Base Sequence Cell Division Chickens DNA Erythrocytes/cytology,enzymology Exons Gene Expression Regulation, Enzymologic Heme/metabolism Humans Molecular Sequence Data Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid Restriction Mapping Sequence Homology, Nucleic Acid Transcription, Genetic
Chemicals
Heme DNA 5-Aminolevulinate Synthetase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Lim K C
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208.
Ishihara H
Riddle R D
Yang Z
Andrews N
Yamamoto M
Engel J D
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/22.7.1226
Published
1994-04-11
Pages
1226-33
Language
English
Region
England
NLM ID
0411011
PMCID
PMC523647
Subset
IM
Grants
NHLBI NIH HHS · HL 24415 · United States
NHLBI NIH HHS · HL 45168 · United States
Databases
GENBANK
S69605
External Links
PubMed source DOI: 10.1093/nar/22.7.1226 PMC Full Text View journal details
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