A genetic polymorphism in the constant region of rabbit b4 kappa chains.

The Journal of experimental medicine ·Vol. 143 ·No. 6 ·1976-06-01 ·Pages 1475-82

Sogn JA, Kindt TJ

Abstract

Amino acid sequence analysis of a b4 light chain from a rabbit homogeneous antistreptococcal antibody revealed the presence of two amino acid substitutions in the constant region not previously reported for these positions. These interchanges, consisting of serine for alanine at position 121 and leucine for glutamine at position 124, were also present in about 30% of the pooled b4 light chains isolated from pooled IgG from the rabbit (4539) that produced the homogeneous antibody. In addition, these interchanges (b4var) were found, always at the same levels, in varying percentages in nonimmune or early immune bleedings from related rabbits in this pedigreed family and could be traced for five generations. The inheritance pattern of b4var was consistent with autosomal codominant inheritance.

MeSH Terms

Amino Acid Sequence Animals Genes Immunoglobulin Light Chains/analysis Immunoglobulin kappa-Chains/analysis Pedigree Polymorphism, Genetic Rabbits

Chemicals

Immunoglobulin Light Chains Immunoglobulin kappa-Chains

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Sogn J A

Kindt T J

References (28)

28 references, click to expand

ALLOTYPIC SPECIFICITIES OF A- AND B-CHAINS OF RABBIT GAMMA GLOBULIN.
Science. 1964 Jul 24;145(3630):403-5 PMID: 14172605
THE ARRANGEMENT OF THE PEPTIDE CHAINS IN GAMMA-GLOBULIN.
Biochem J. 1963 Aug;88:220-8 PMID: 14063859
Allotypy of rabbit serum proteins. I. Immuno-chemical analysis leading to the individualization of seven main allotypes.
J Exp Med. 1960 Jul 1;112:107-24 PMID: 13731716
Idiotypic cross-reactions among anti-streptococcal antibodies in an inbred rabbit population.
Scand J Immunol. 1974;3(4):483-90 PMID: 4852349
The search for antibodies with molecular uniformity.
Adv Immunol. 1970;12:1-56 PMID: 4098533
Specific cleavage between variable and constant domains of rabbit antibody light chains by dilute acid hydrolysis.
Biochemistry. 1972 Dec 19;11(26):4974-7 PMID: 4404720
Amino acid sequence of rabbit pneumococcal antibody. I. Light-chain cysteine-containing peptides.
Biochemistry. 1972 Dec 19;11(26):4978-85 PMID: 4118103
Serologic identity of the b4 allotypic determinants present on homogeneous rabbit light chains with different N-terminal amino acid sequences.
J Immunol. 1972 Oct;109(4):735-41 PMID: 4116357
A rabbit with the allotypic phenotype: ala2a3 b4b5b6.
J Immunol. 1974 Oct;113(4):1313-8 PMID: 4137513
Amino acid sequence of the N-terminal 139 residues of light chain derived from a homogeneous rabbit antibody.
Biochem J. 1974 Jul;141(1):1-13 PMID: 4141892
Comparison of the amino acid sequences of the variable regions of light chains derived from two homogeneous rabbit anti-pneumococcal antibodies.
Biochem J. 1974 Jul;141(1):15-25 PMID: 4156171
Rabbit immunoglobulin allotypes: structure, immunology, and genetics.
Adv Immunol. 1975;21:35-86 PMID: 50001
Allelic selection of light chain allotypes by antibodies raised in heterozygous rabbits.
J Immunol. 1974 Feb;112(2):601-6 PMID: 4591975
Genetics and regulation of immunoglobulin allotypes.
Med Clin North Am. 1972 Mar;56(2):557-75 PMID: 4553197
Amino-acid sequence of an allotype b4 light chain from a rabbit antibody to streptococcal carbohydrate.
Proc Natl Acad Sci U S A. 1974 May;71(5):1995-8 PMID: 4525311
Thin-layer chromatography of sub-nanomole amounts of phenylthiohydantoin (PTH) amino acids on polyamide sheets.
Anal Biochem. 1973 Jun;53(2):624-8 PMID: 4716391
Sequence variations in b9 light chains as potential V region genetic markers.
J Immunol. 1973 Dec;111(6):1755-64 PMID: 4750866
Amino acid sequence of the light chain derived from a rabbit anti-p-azobenzoate antibody of restricted heterogeneity.
Biochem Biophys Res Commun. 1973 Aug 21;53(4):1122-9 PMID: 4748811
Recovery of large amounts of antibody from immunized rabbits by multiple non-surgical exchange transfusions.
J Immunol. 1973 Mar;110(3):862-6 PMID: 4688922
Subtypes of rabbit kappa light polypeptide chains associated with the beta locus.
Biochemistry. 1969 Jul;8(7):2712-8 PMID: 4980104
Separation of two types of light polypeptide chains from rabbit gamma-globulins.
Eur J Biochem. 1969 Dec;11(2):370-5 PMID: 4982821
The variability of human lambda-chain constant regions and some relationships to V-regions sequences.
Immunochemistry. 1976 Feb;13(2):149-55 PMID: 816731
The organization, expression, and evolution of antibody genes and other multigene families.
Annu Rev Genet. 1975;9:305-53 PMID: 813561
Quantitative determination of allotypic and idiotypic markers with antisera coupled to N-hydroxysuccinimide-activated sepharose.
J Immunol. 1975 Jan;114(1 Pt 1):51-4 PMID: 1167562
Nonallelic behavior of rabbit variable-region allotypes.
J Exp Med. 1975 Jun 1;141(6):1448-52 PMID: 1127384
Identification and genetic control of two rabbit immunoglobulin allotypes at a second light chain locus, the c locus.
J Immunol. 1969 Dec;103(6):1159-67 PMID: 5361561
Analysis of amino acid phenylthiohydantoins by gas chromatography.
J Biol Chem. 1969 Oct 25;244(20):5597-607 PMID: 5348603
Hydrolysis of phenylthiohydantoins of amino acids.
Biochem Biophys Res Commun. 1964;14:399-403 PMID: 5836532

Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.143.6.1475

Published

1976-06-01

Pages

1475-82

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2190215

Subset

External Links

PubMed source DOI: 10.1084/jem.143.6.1475 PMC Full Text View journal details

Analysis Services

A genetic polymorphism in the constant region of rabbit b4 kappa chains.

Contact

WeChat Official Account