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PMID: 8187766 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Requirement for the MAP kinase kinase/MAP kinase cascade in Xenopus oocyte maturation.

The EMBO journal ·Vol. 13 ·No. 9 ·1994-05-01 ·Pages 2131-8

Kosako H, Gotoh Y, Nishida E

Abstract

MAP kinase kinase (MAPKK) has been identified as a protein factor that can induce phosphorylation and activation of inactive MAP kinase in vitro. In this study, we produced an anti-Xenopus MAPKK antibody that can specifically inhibit Xenopus MAPKK activity in vitro. Microinjection of this antibody into immature oocytes prevented progesterone-induced MAP kinase activation. Moreover, progesterone-induced histone H1 kinase activation and germinal vesicle breakdown (GVBD) were inhibited in the oocytes injected previously with this antibody. Furthermore, when a bacterially expressed Mos was introduced into immature oocytes, Mos-induced MAP kinase activation and GVBD were blocked in the oocytes injected with the anti-MAPKK antibody. These results show that MAPKK is responsible for the activation of MAP kinase in vivo and that the MAPKK/MAP kinase cascade plays a pivotal role in the MPF activation during the oocyte maturation process.

MeSH Terms
Animals Antibodies, Monoclonal Antibody Specificity Cell-Free System Enzyme Activation Ethers, Cyclic/pharmacology Microinjections Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase Kinases Okadaic Acid Oocytes/cytology,enzymology Oogenesis Progesterone/antagonists & inhibitors,physiology Protein Kinases/immunology,metabolism Protein Serine-Threonine Kinases/metabolism Protein-Tyrosine Kinases/metabolism Signal Transduction Xenopus
Chemicals
Antibodies, Monoclonal Ethers, Cyclic Okadaic Acid Progesterone Protein Kinases Protein-Tyrosine Kinases Protein Serine-Threonine Kinases Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase Kinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kosako H
Department of Genetics and Molecular Biology, Kyoto University, Japan.
Gotoh Y
Nishida E
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-05-01
Pages
2131-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395066
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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