The nucleotide sequence of cysteine transfer ribonucleic acid from baker's yeast. Identification of the products from partial degradation of the molecule and derivation of the complete sequence.

The Biochemical journal ·Vol. 153 ·No. 2 ·1976-02-01 ·Pages 447-54

Holness NJ, Atfield G

Abstract

1. A series of large oligonucleotide fragments derived from tRNA Cys, were separated chromatographically and the sequence of each was deduced by examination of the products of digestion with pancreatic and T1 ribonucleases. 2. The location of the specific cleavage points in the nucleotide chain was similar to that produced by brief treatment with pancreatic ribonuclease. 3. The fragments could be arranged into two alternative sequences. The correct sequence was deduced by the sequential removal and identification of the first nine nucleotides from the 3'-end of the terminal half of the molecules.

MeSH Terms

Base Sequence Chromatography, DEAE-Cellulose Cysteine Pancreas/enzymology RNA, Transfer/analysis Ribonuclease T1 Ribonucleases Saccharomyces cerevisiae/analysis

Chemicals

RNA, Transfer Ribonucleases Ribonuclease T1 Cysteine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Holness N J

Atfield G

References (30)

30 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1530447

Published

1976-02-01

Pages

447-54

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1172592

Subset

Databases

GENBANK

K00180

External Links

PubMed source DOI: 10.1042/bj1530447 PMC Full Text View journal details

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The nucleotide sequence of cysteine transfer ribonucleic acid from baker's yeast. Identification of the products from partial degradation of the molecule and derivation of the complete sequence.

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