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PMID: 8190063 Published · ppublish English Journal Article

High fidelity extrachromosomal recombination and gene targeting in plants.

Molecular & general genetics : MGG ·Vol. 243 ·No. 1 ·1994-04-00 ·Pages 106-11

Hrouda M, Paszkowski J

Abstract

The precision of extrachromosomal homologous recombination and gene targeting in plant cells was investigated. Recombination was directed to introns of selectable marker genes where potential changes could persist without affecting the function and therefore the selectability of the genes. Approximately 9 kb of crossover regions was rescued and sequenced. Changes were detected at a frequency below one point mutation per 1000 bp, indicating that extrachromosomal recombination and gene targeting both appear to occur with high fidelity.

MeSH Terms
Crossing Over, Genetic Genetic Markers Plant Cells Plants/genetics Protoplasts Recombination, Genetic
Chemicals
Genetic Markers
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hrouda M
Research Institute for Corp Production, Praha, Czech Republic.
Paszkowski J
References (35)
35 references, click to expand
  1. PCR amplification of long DNA fragments.
    Nucleic Acids Res. 1992 Feb 11;20(3):623 PMID: 1741307
  2. High-fidelity gene targeting in embryonic stem cells by using sequence replacement vectors.
    Mol Cell Biol. 1992 Jul;12 (7):2919-23 PMID: 1620105
  3. Expression in transgenic tobacco of the bacterial neomycin phosphotransferase gene modified by intron insertions of various sizes.
    Plant Mol Biol. 1992 Aug;19(5):825-36 PMID: 1322741
  4. DNA recombination during PCR.
    Nucleic Acids Res. 1990 Apr 11;18(7):1687-91 PMID: 2186361
  5. Stable transformation of tobacco by electroporation: evidence for plasmid concatenation.
    Proc Natl Acad Sci U S A. 1986 Aug;83(15):5602-6 PMID: 3016708
  6. Rapid isolation of high molecular weight plant DNA.
    Nucleic Acids Res. 1980 Oct 10;8(19):4321-5 PMID: 7433111
  7. Streptomycin-resistant plants from callus culture of haploid tobacco.
    Nat New Biol. 1973 Jul 4;244(131):29-30 PMID: 4515911
  8. Intermolecular homologous recombination in plants.
    Mol Cell Biol. 1990 Feb;10(2):492-500 PMID: 2300050
  9. Two T-DNA's co-transformed intoBrassica napus by a doubleAgrobacterium tumefaciens infection are mainly integrated at the same locus.
    Theor Appl Genet. 1991 Sep;82(3):257-63 PMID: 24213168
  10. A transient assay in plant cells reveals a positive correlation between extrachromosomal recombination rates and length of homologous overlap.
    Nucleic Acids Res. 1991 May 25;19(10):2693-700 PMID: 2041745
  11. Hybrid genes in the analysis of transformation conditions : I. Setting up a simple method for direct gene transfer in plant protoplasts.
    Plant Mol Biol. 1987 Sep;8(5):363-73 PMID: 24301258
  12. Targeted mutation of the Hprt gene in mouse embryonic stem cells.
    Proc Natl Acad Sci U S A. 1988 Nov;85(22):8583-7 PMID: 3186749
  13. Introduction of homologous DNA sequences into mammalian cells induces mutations in the cognate gene.
    Nature. 1986 Nov 6-12;324(6092):34-8 PMID: 3785372
  14. Target frequency and integration pattern for insertion and replacement vectors in embryonic stem cells.
    Mol Cell Biol. 1991 Sep;11(9):4509-17 PMID: 1875936
  15. Fidelity of targeted recombination in human fibroblasts and murine embryonic stem cells.
    Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):8067-71 PMID: 1896453
  16. Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase.
    Biochemistry. 1988 Aug 9;27(16):6008-13 PMID: 2847780
  17. Gene targeting in Arabidopsis thaliana.
    Mol Gen Genet. 1992 Jan;231(2):186-93 PMID: 1310519
  18. Single-stranded DNA as a recombination substrate in plants as assessed by stable and transient recombination assays.
    Mol Cell Biol. 1992 Jan;12(1):329-36 PMID: 1729608
  19. Heteroduplex-induced mutagenesis in mammalian cells.
    Nucleic Acids Res. 1988 Mar 25;16(5):2313-22 PMID: 2833731
  20. Reexamination of gene targeting frequency as a function of the extent of homology between the targeting vector and the target locus.
    Mol Cell Biol. 1992 Aug;12(8):3365-71 PMID: 1321331
  21. Transformation of yeast.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929-33 PMID: 347451
  22. Targeted correction of a major histocompatibility class II E alpha gene by DNA microinjected into mouse eggs.
    Proc Natl Acad Sci U S A. 1989 Sep;86(18):7087-91 PMID: 2506546
  23. Disruption of the Dictyostelium myosin heavy chain gene by homologous recombination.
    Science. 1987 May 29;236(4805):1086-91 PMID: 3576222
  24. Homologous recombination between plasmid DNA molecules in maize protoplasts.
    Mol Gen Genet. 1991 Nov;230(1-2):209-18 PMID: 1745230
  25. Gene targeting in plants.
    EMBO J. 1988 Dec 20;7(13):4021-6 PMID: 16453864
  26. Highly efficient gene targeting in embryonic stem cells through homologous recombination with isogenic DNA constructs.
    Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):5128-32 PMID: 1594621
  27. Liposome-mediated transformation of tobacco mesophyll protoplasts by an Escherichia coli plasmid.
    EMBO J. 1985 Nov;4(11):2731-7 PMID: 3905385
  28. Insertion of DNA sequences into the human chromosomal beta-globin locus by homologous recombination.
    Nature. 1985 Sep 19-25;317(6034):230-4 PMID: 2995814
  29. Extrachromosomal homologous recombination and gene targeting in plant cells after Agrobacterium mediated transformation.
    EMBO J. 1990 Oct;9(10):3077-84 PMID: 2209538
  30. Homologous recombination in plant cells after Agrobacterium-mediated transformation.
    Plant Cell. 1990 May;2(5):415-25 PMID: 2152167
  31. High frequency targeting of genes to specific sites in the mammalian genome.
    Cell. 1986 Feb 14;44(3):419-28 PMID: 3002636
  32. The AU-rich sequences present in the introns of plant nuclear pre-mRNAs are required for splicing.
    Cell. 1989 Aug 11;58(3):473-83 PMID: 2758463
  33. Homologous recombination in mammalian cells.
    Annu Rev Genet. 1989;23:199-225 PMID: 2694931
  34. Recombination of selectable marker DNA in Nicotiana tabacum.
    DNA. 1987 Jun;6(3):245-53 PMID: 3036454
  35. The role and fate of DNA ends for homologous recombination in embryonic stem cells.
    Mol Cell Biol. 1992 Jun;12(6):2464-74 PMID: 1588950
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00283882
Published
1994-04-00
Pages
106-11
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00283882
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