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PMID: 8195092 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Stress induction of clpC in Bacillus subtilis and its involvement in stress tolerance.

Journal of bacteriology ·Vol. 176 ·No. 11 ·1994-06-00 ·Pages 3360-7

Krüger E, Völker U, Hecker M

Abstract

A member of the clpC subfamily of stress response-related Clp ATPases was cloned from Bacillus subtilis. The B. subtilis clpC gene was induced in response to various stresses, including heat shock. Its product was identified as a general stress protein (Gsp12) described previously. A dramatic increase in the amount of clpC mRNA immediately after exposure to multiple stresses suggested regulation on a transcriptional level. Induction by heat shock was independent of the alternative sigma factor SigB, indicating a new mechanism of heat shock induction in B. subtilis. A clpC insertional mutant had an impaired tolerance for heat shock and salt stress. Furthermore, the mutation triggered the formation of elongated cells, a phenomenon particularly pronounced during stress.

Related Genes
clpC
MeSH Terms
Adaptation, Biological/genetics Amino Acid Sequence Bacillus subtilis/cytology,enzymology,genetics,growth & development Bacterial Proteins/genetics Base Sequence Cell Division Cloning, Molecular Gene Expression Regulation, Bacterial Heat-Shock Proteins/genetics Molecular Sequence Data Mutagenesis, Insertional Oligonucleotides Polymerase Chain Reaction RNA, Messenger/analysis Sequence Analysis, DNA
Chemicals
Bacterial Proteins ClpC protein, Bacteria Heat-Shock Proteins Oligonucleotides RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Krüger E
Institut für Mikrobiologie und Molekularbiologie, Ernst-Moritz-Arndt-Universität, Greifswald, Germany.
Völker U
Hecker M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.11.3360-3367.1994
Published
1994-06-00
Pages
3360-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205508
Subset
IM
Databases
GENBANK
X75930
External Links
PubMed source DOI: 10.1128/jb.176.11.3360-3367.1994 PMC Full Text View journal details
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