Home LiteratureArticle Details
PMID: 8195384 Published · ppublish English Journal Article

Isolation of Borrelia burgdorferi from biopsy specimens taken from healthy-looking skin of patients with Lyme borreliosis.

Journal of clinical microbiology ·Vol. 32 ·No. 3 ·1994-03-00 ·Pages 715-20

Kuiper H, van Dam AP, Spanjaard L, de Jongh BM, Widjojokusumo A, Ramselaar TC, Cairo I, Vos K, Dankert J

Abstract

Erythematous skin lesions due to infection with Borrelia burgdorferi will often disappear without antibiotic treatment. The aim of the study was to assess whether after disappearance of the erythematous skin lesion B. burgdorferi is still present in the healthy-looking skin of untreated patients. In six patients, a skin biopsy specimen was taken at the site of a previous erythematous skin lesion 1 to 6 months after disappearance of the lesion. Four of them presented with early disseminated Lyme borreliosis. In one additional patient with early disseminated Lyme borreliosis, the site of a previous tick bite was biopsied. None of these patients had been treated with antibiotics before presentation. The cultures of the skin biopsy specimens of the seven patients showed growth of Borrelia species. By rRNA gene restriction analysis and genospecies-specific PCR, six isolates were classified as Borrelia garinii and one as Borrelia group VS461. These results show that B. burgdorferi can still be cultured from the skin after disappearance of the erythematous skin lesion or at the site of a previous tick bite.

MeSH Terms
Adolescent Adult Antibodies, Bacterial/blood,cerebrospinal fluid Base Sequence Borrelia burgdorferi Group/genetics,immunology,isolation & purification Child DNA Primers/genetics DNA, Bacterial/genetics DNA, Ribosomal/genetics Erythema Chronicum Migrans/microbiology Female Humans Lyme Disease/diagnosis,immunology,microbiology Male Middle Aged Molecular Sequence Data Polymerase Chain Reaction Skin/microbiology Time Factors
Chemicals
Antibodies, Bacterial DNA Primers DNA, Bacterial DNA, Ribosomal
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Kuiper H
Department of Medical Microbiology, Academic Medical Centre, University Hospital, University of Amsterdam, The Netherlands.
van Dam A P
Spanjaard L
de Jongh B M
Widjojokusumo A
Ramselaar T C
Cairo I
Vos K
Dankert J
References (35)
35 references, click to expand
  1. Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity.
    Anal Biochem. 1981 Nov 1;117(2):307-10 PMID: 6172996
  2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  3. Spirochetes isolated from the blood of two patients with Lyme disease.
    N Engl J Med. 1983 Mar 31;308(13):740-2 PMID: 6828119
  4. The early clinical manifestations of Lyme disease.
    Ann Intern Med. 1983 Jul;99(1):76-82 PMID: 6859726
  5. Clinical manifestations of erythema chronicum migrans Afzelius in 161 patients. A comparison with Lyme disease.
    Acta Derm Venereol. 1985;65(1):43-52 PMID: 2578705
  6. Borrelia burgdorferi in joint fluid in chronic Lyme arthritis.
    Ann Intern Med. 1986 Jun;104(6):798-800 PMID: 3518562
  7. European Borrelia burgdorferi isolated from humans and ticks culture conditions and antibiotic susceptibility.
    Zentralbl Bakteriol Mikrobiol Hyg A. 1986 Dec;263(1-2):112-8 PMID: 3577473
  8. Erythema chronicum migrans Afzelius in Sweden. A study on 231 patients.
    Zentralbl Bakteriol Mikrobiol Hyg A. 1986 Dec;263(1-2):229-36 PMID: 3577482
  9. Microbiological findings in erythema (chronicum) migrans and related disorders.
    Zentralbl Bakteriol Mikrobiol Hyg A. 1986 Dec;263(1-2):237-52 PMID: 3577483
  10. Clinical manifestations of acrodermatitis chronica atrophicans in 50 Swedish patients.
    Zentralbl Bakteriol Mikrobiol Hyg A. 1986 Dec;263(1-2):253-61 PMID: 3577484
  11. Bannwarth's syndrome and the enlarged neurological spectrum of arthropod-borne borreliosis.
    Zentralbl Bakteriol Mikrobiol Hyg A. 1987 Feb;263(3):343-7 PMID: 3591086
  12. Isolation of Borrelia spirochetes from patients in Texas.
    J Clin Microbiol. 1987 Jul;25(7):1148-50 PMID: 3611307
  13. Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools.
    Ann Inst Pasteur Microbiol. 1986 Sep-Oct;137B(2):165-75 PMID: 2446639
  14. Measurement of antibodies to the Borrelia burgdorferi flagellum improves serodiagnosis in Lyme disease.
    J Clin Microbiol. 1988 Feb;26(2):338-46 PMID: 3343329
  15. Cultivation of Borrelia burgdorferi from joint fluid three months after treatment of facial palsy due to Lyme borreliosis.
    J Infect Dis. 1988 Oct;158(4):905-6 PMID: 3171237
  16. Lyme disease.
    N Engl J Med. 1989 Aug 31;321(9):586-96 PMID: 2668764
  17. Isolation of Borrelia burgdorferi from the blood of seven patients with Lyme disease.
    Am J Med. 1990 Jan;88(1):21-6 PMID: 2294761
  18. Survival of Borrelia burgdorferi in antibiotically treated patients with Lyme borreliosis.
    Infection. 1989 Nov-Dec;17(6):355-9 PMID: 2613324
  19. Cultivation and characterization of spirochetes from cerebrospinal fluid of patients with Lyme borreliosis.
    J Clin Microbiol. 1990 Mar;28(3):473-9 PMID: 2324275
  20. Comments on the course and classification of Lyme borreliosis.
    Scand J Infect Dis Suppl. 1991;77:41-3 PMID: 1947810
  21. Cultivation of Borrelia burgdorferi from erythema migrans lesions and perilesional skin.
    J Clin Microbiol. 1992 Feb;30(2):359-61 PMID: 1537904
  22. Species-specific identification of and distinction between Borrelia burgdorferi genomic groups by using 16S rRNA-directed oligonucleotide probes.
    J Clin Microbiol. 1992 Mar;30(3):628-32 PMID: 1372620
  23. Identification of a third genomic group of Borrelia burgdorferi through signature nucleotide analysis and 16S rRNA sequence determination.
    J Gen Microbiol. 1992 Mar;138(3):533-6 PMID: 1593264
  24. The clinical and epidemiological profile of Lyme neuroborreliosis in Denmark 1985-1990. A prospective study of 187 patients with Borrelia burgdorferi specific intrathecal antibody production.
    Brain. 1992 Apr;115 ( Pt 2):399-423 PMID: 1606475
  25. Failure of Borrelia burgdorferi to survive in the skin of patients with antibiotic-treated Lyme disease.
    J Am Acad Dermatol. 1992 Jul;27(1):34-7 PMID: 1619074
  26. Fibroblasts protect the Lyme disease spirochete, Borrelia burgdorferi, from ceftriaxone in vitro.
    J Infect Dis. 1992 Aug;166(2):440-4 PMID: 1634816
  27. The Th1/Th2-like switch in syphilitic infection: is it detrimental?
    Infect Immun. 1992 Sep;60(9):3475-9 PMID: 1386838
  28. Delineation of Borrelia burgdorferi sensu stricto, Borrelia garinii sp. nov., and group VS461 associated with Lyme borreliosis.
    Int J Syst Bacteriol. 1992 Jul;42(3):378-83 PMID: 1380285
  29. Development of polymerase chain reaction primer sets for diagnosis of Lyme disease and for species-specific identification of Lyme disease isolates by 16S rRNA signature nucleotide analysis.
    J Clin Microbiol. 1992 Nov;30(11):2830-4 PMID: 1280643
  30. Diagnosis of early Lyme disease by polymerase chain reaction amplification and culture of skin biopsies from erythema migrans lesions.
    J Clin Microbiol. 1992 Dec;30(12):3082-8 PMID: 1452688
  31. Isolation of Borrelia burgdorferi from skin biopsy specimens of patients with erythema migrans.
    Am J Clin Pathol. 1993 Jan;99(1):104-7 PMID: 8422007
  32. Invasion of human skin fibroblasts by the Lyme disease spirochete, Borrelia burgdorferi.
    J Infect Dis. 1993 May;167(5):1074-81 PMID: 8486939
  33. Failure to isolate Borrelia burgdorferi after antimicrobial therapy in culture-documented Lyme borreliosis associated with erythema migrans: report of a prospective study.
    Am J Med. 1993 Jun;94(6):583-8 PMID: 8506882
  34. Different genospecies of Borrelia burgdorferi are associated with distinct clinical manifestations of Lyme borreliosis.
    Clin Infect Dis. 1993 Oct;17(4):708-17 PMID: 7903558
  35. The spirochetal etiology of Lyme disease.
    N Engl J Med. 1983 Mar 31;308(13):733-40 PMID: 6828118
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.32.3.715-720.1994
Published
1994-03-00
Pages
715-20
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC263113
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.32.3.715-720.1994 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2