Home LiteratureArticle Details
PMID: 8197184 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Qualitative changes in the subunit composition of kappa B-binding complexes during murine B-cell differentiation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 11 ·1994-05-24 ·Pages 5056-60

Miyamoto S, Schmitt MJ, Verma IM

Abstract

We report here that the major kappa B-binding complex in murine mature B cells is composed of a p50-Rel heterodimer, whereas the major inducible form in pre-B cells is a p50-p65 heterodimer. Treatment of a pre-B-cell line with lipopolysaccharide changes the subunit composition of kappa B-binding complexes from p50-p65 to p50-Rel. This change is preceded by the enhanced Rel expression and correlates with the expression of the gene for the immunoglobin kappa light chain. The heterodimeric p50-Rel complex binds to the intronic enhancer-kappa B site in the immunoglobulin kappa light chain gene at least 20-fold more stably than does the p50-p65 dimer. These data support a model in which augmentation of Rel expression during the differentiation of pre-B cells to mature B cells leads to an exchange of kappa B-binding subunits resulting in the transcriptional activation of the gene for the immunoglobulin kappa light chain.

MeSH Terms
Amino Acid Sequence Animals B-Lymphocytes/cytology,metabolism Base Sequence Cell Differentiation Cell Line DNA Lipopolysaccharides/pharmacology Mice Molecular Sequence Data NF-kappa B/metabolism NF-kappa B p50 Subunit Protein Binding Transcription Factor RelA
Chemicals
Lipopolysaccharides NF-kappa B NF-kappa B p50 Subunit Transcription Factor RelA DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Miyamoto S
Molecular Biology and Virology Laboratory, Salk Institute, San Diego, CA 92186-5800.
Schmitt M J
Verma I M
References (34)
34 references, click to expand
  1. Diverse molecular mechanisms of inhibition of NF-kappa B/DNA binding complexes by I kappa B proteins.
    Gene Expr. 1993;3(2):135-50 PMID: 8268718
  2. rel/NF-kappa B nuclear complexes that bind kB sites in the murine c-rel promoter are required for constitutive c-rel transcription in B-cells.
    Cell Growth Differ. 1993 Sep;4(9):731-43 PMID: 8241021
  3. Murine B cell leukemia line with inducible surface immunoglobulin expression.
    J Immunol. 1978 Aug;121(2):641-7 PMID: 308074
  4. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  5. Nucleic acid sequences of the oncogene v-rel in reticuloendotheliosis virus strain T and its cellular homolog, the proto-oncogene c-rel.
    J Virol. 1984 Oct;52(1):172-82 PMID: 6090694
  6. Multiple nuclear factors interact with the immunoglobulin enhancer sequences.
    Cell. 1986 Aug 29;46(5):705-16 PMID: 3091258
  7. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  8. Activation of DNA-binding activity in an apparently cytoplasmic precursor of the NF-kappa B transcription factor.
    Cell. 1988 Apr 22;53(2):211-7 PMID: 3129195
  9. I kappa B: a specific inhibitor of the NF-kappa B transcription factor.
    Science. 1988 Oct 28;242(4878):540-6 PMID: 3140380
  10. The DNA binding subunit of NF-kappa B is identical to factor KBF1 and homologous to the rel oncogene product.
    Cell. 1990 Sep 7;62(5):1007-18 PMID: 2203531
  11. Cloning of the p50 DNA binding subunit of NF-kappa B: homology to rel and dorsal.
    Cell. 1990 Sep 7;62(5):1019-29 PMID: 2203532
  12. The murine c-rel proto-oncogene encodes two mRNAs the expression of which is modulated by lymphoid stimuli.
    Oncogene Res. 1990;5(4):245-54 PMID: 2204017
  13. The v-rel oncogene encodes a kappa B enhancer binding protein that inhibits NF-kappa B function.
    Cell. 1990 Nov 16;63(4):803-14 PMID: 2225078
  14. Cloning of a mitogen-inducible gene encoding a kappa B DNA-binding protein with homology to the rel oncogene and to cell-cycle motifs.
    Nature. 1990 Nov 1;348(6296):76-80 PMID: 2234062
  15. Cloning of the DNA-binding subunit of human nuclear factor kappa B: the level of its mRNA is strongly regulated by phorbol ester or tumor necrosis factor alpha.
    Proc Natl Acad Sci U S A. 1991 Feb 1;88(3):966-70 PMID: 1992489
  16. DNA binding and I kappa B inhibition of the cloned p65 subunit of NF-kappa B, a rel-related polypeptide.
    Cell. 1991 Mar 8;64(5):961-9 PMID: 2001591
  17. Isolation of a rel-related human cDNA that potentially encodes the 65-kD subunit of NF-kappa B.
    Science. 1991 Mar 22;251(5000):1490-3 PMID: 2006423
  18. c-rel activates but v-rel suppresses transcription from kappa B sites.
    Proc Natl Acad Sci U S A. 1991 May 1;88(9):3715-9 PMID: 2023921
  19. Cloning of an NF-kappa B subunit which stimulates HIV transcription in synergy with p65.
    Nature. 1991 Aug 22;352(6337):733-6 PMID: 1876189
  20. Immunoglobulin gene transcription.
    Annu Rev Immunol. 1991;9:373-98 PMID: 1910683
  21. Regulation and a possible stage-specific function of Oct-2 during pre-B-cell differentiation.
    Mol Cell Biol. 1991 Oct;11(10):4885-94 PMID: 1922024
  22. B cell lymphoma-associated chromosomal translocation involves candidate oncogene lyt-10, homologous to NF-kappa B p50.
    Cell. 1991 Dec 20;67(6):1075-87 PMID: 1760839
  23. Functional characterization of the NF-kappa B p65 transcriptional activator and an alternatively spliced derivative.
    Mol Cell Biol. 1992 Feb;12(2):444-54 PMID: 1732726
  24. RelB, a new Rel family transcription activator that can interact with p50-NF-kappa B.
    Mol Cell Biol. 1992 Feb;12(2):674-84 PMID: 1732739
  25. A novel mitogen-inducible gene product related to p50/p105-NF-kappa B participates in transactivation through a kappa B site.
    Mol Cell Biol. 1992 Feb;12(2):685-95 PMID: 1531086
  26. Direct association of pp40/I kappa B beta with rel/NF-kappa B transcription factors: role of ankyrin repeats in the inhibition of DNA binding activity.
    Proc Natl Acad Sci U S A. 1992 May 15;89(10):4333-7 PMID: 1533932
  27. Molecular cloning and characterization of a novel Rel/NF-kappa B family member displaying structural and functional homology to NF-kappa B p50/p105.
    DNA Cell Biol. 1992 Sep;11(7):523-37 PMID: 1388725
  28. NF-kappa B and Rel: participants in a multiform transcriptional regulatory system.
    Int Rev Cytol. 1993;143:1-62 PMID: 8449662
  29. NF-kappa B controls expression of inhibitor I kappa B alpha: evidence for an inducible autoregulatory pathway.
    Science. 1993 Mar 26;259(5103):1912-5 PMID: 8096091
  30. Mutual regulation of the transcriptional activator NF-kappa B and its inhibitor, I kappa B-alpha.
    Proc Natl Acad Sci U S A. 1993 Mar 15;90(6):2532-6 PMID: 8460169
  31. The p65 subunit of NF-kappa B regulates I kappa B by two distinct mechanisms.
    Genes Dev. 1993 Jul;7(7A):1266-76 PMID: 8319912
  32. Common structural constituents confer I kappa B activity to NF-kappa B p105 and I kappa B/MAD-3.
    EMBO J. 1993 Jul;12(7):2781-8 PMID: 8334994
  33. Distinct NF-kappa B/Rel transcription factors are responsible for tissue-specific and inducible gene activation.
    Nature. 1993 Oct 21;365(6448):767-70 PMID: 7692309
  34. Photochemical attachment of lac repressor to bromodeoxyuridine-substituted lac operator by ultraviolet radiation.
    Proc Natl Acad Sci U S A. 1974 Mar;71(3):947-51 PMID: 4522804
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.11.5056
Published
1994-05-24
Pages
5056-60
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC43930
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.91.11.5056 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2