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PMID: 8202544 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

D-MEF2: a MADS box transcription factor expressed in differentiating mesoderm and muscle cell lineages during Drosophila embryogenesis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 12 ·1994-06-07 ·Pages 5662-6

Lilly B, Galewsky S, Firulli AB, Schulz RA, Olson EN

Abstract

The myocyte enhancer factor (MEF) 2 family of transcription factors has been implicated in the regulation of muscle transcription in vertebrates. We have cloned a protein from Drosophila, termed D-MEF2, that shares extensive amino acid homology with the MADS (MCM1, Agamous, Deficiens, and serum-response factor) domains of the vertebrate MEF2 proteins. D-mef2 gene expression is first detected during Drosophila embryogenesis within mesodermal precursor cells prior to specification of the somatic and visceral muscle lineages. Expression of D-mef2 is dependent on the mesodermal determinants twist and snail but independent of the homeobox-containing gene tinman, which is required for visceral muscle and heart development. D-mef2 expression precedes that of the MyoD homologue, nautilus, and, in contrast to nautilus, D-mef2 appears to be expressed in all somatic and visceral muscle cell precursors. Its temporal and spatial expression patterns suggest that D-mef2 may play an important role in commitment of mesoderm to myogenic lineages.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Binding Sites Cell Compartmentation Cloning, Molecular DNA, Complementary/genetics DNA-Binding Proteins/genetics,metabolism Drosophila Proteins Drosophila melanogaster/embryology,genetics Enhancer Elements, Genetic Gene Expression Genes, Insect MEF2 Transcription Factors Mesoderm/metabolism Molecular Sequence Data Muscles/embryology Myogenic Regulatory Factors RNA, Messenger/genetics,metabolism Sequence Alignment Sequence Homology, Amino Acid Transcription Factors/genetics
Chemicals
DNA, Complementary DNA-Binding Proteins Drosophila Proteins MEF2 Transcription Factors Mef2 protein, Drosophila Myogenic Regulatory Factors RNA, Messenger Transcription Factors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lilly B
Department of Biochemistry and Molecular Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Galewsky S
Firulli A B
Schulz R A
Olson E N
References (19)
19 references, click to expand
  1. The regulation of myogenin gene expression during the embryonic development of the mouse.
    Genes Dev. 1993 Jul;7(7A):1277-89 PMID: 8391506
  2. Myocyte enhancer factor (MEF) 2C: a tissue-restricted member of the MEF-2 family of transcription factors.
    Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5282-6 PMID: 8506376
  3. tinman and bagpipe: two homeo box genes that determine cell fates in the dorsal mesoderm of Drosophila.
    Genes Dev. 1993 Jul;7(7B):1325-40 PMID: 8101173
  4. Binding of TFIID and MEF2 to the TATA element activates transcription of the Xenopus MyoDa promoter.
    Mol Cell Biol. 1994 Jan;14(1):686-99 PMID: 8264638
  5. A fourth human MEF2 transcription factor, hMEF2D, is an early marker of the myogenic lineage.
    Development. 1993 Aug;118(4):1095-106 PMID: 8269842
  6. A Mef2 gene that generates a muscle-specific isoform via alternative mRNA splicing.
    Mol Cell Biol. 1994 Mar;14(3):1647-56 PMID: 8114702
  7. Mef2 gene expression marks the cardiac and skeletal muscle lineages during mouse embryogenesis.
    Development. 1994 May;120(5):1251-63 PMID: 8026334
  8. The gene tinman is required for specification of the heart and visceral muscles in Drosophila.
    Development. 1993 Jul;118(3):719-29 PMID: 7915669
  9. A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback.
    Chromosoma. 1989 Aug;98(2):81-5 PMID: 2476281
  10. A new myocyte-specific enhancer-binding factor that recognizes a conserved element associated with multiple muscle-specific genes.
    Mol Cell Biol. 1989 Nov;9(11):5022-33 PMID: 2601707
  11. MyoD family: a paradigm for development?
    Genes Dev. 1990 Sep;4(9):1454-61 PMID: 2253873
  12. Expression of a MyoD family member prefigures muscle pattern in Drosophila embryos.
    Genes Dev. 1990 Dec;4(12A):2086-97 PMID: 2176634
  13. The Drosophila homologue of vertebrate myogenic-determination genes encodes a transiently expressed nuclear protein marking primary myogenic cells.
    Proc Natl Acad Sci U S A. 1991 May 1;88(9):3782-6 PMID: 1902570
  14. Human SRF-related proteins: DNA-binding properties and potential regulatory targets.
    Genes Dev. 1991 Dec;5(12A):2327-41 PMID: 1748287
  15. Analysis of the myogenin promoter reveals an indirect pathway for positive autoregulation mediated by the muscle-specific enhancer factor MEF-2.
    Mol Cell Biol. 1992 Sep;12(9):3665-77 PMID: 1324403
  16. Human myocyte-specific enhancer factor 2 comprises a group of tissue-restricted MADS box transcription factors.
    Genes Dev. 1992 Sep;6(9):1783-98 PMID: 1516833
  17. Muscle-specific expression of SRF-related genes in the early embryo of Xenopus laevis.
    EMBO J. 1992 Dec;11(13):4981-91 PMID: 1281451
  18. hMEF2C gene encodes skeletal muscle- and brain-specific transcription factors.
    Mol Cell Biol. 1993 Apr;13(4):2564-77 PMID: 8455629
  19. Separable regulatory elements governing myogenin transcription in mouse embryogenesis.
    Science. 1993 Jul 9;261(5118):215-8 PMID: 8392225
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.12.5662
Published
1994-06-07
Pages
5662-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC44056
Subset
IM
Grants
NIAMS NIH HHS · AR39849 · United States
NIAMS NIH HHS · AR40339 · United States
Databases
GENBANK
U03292, X83527
External Links
PubMed source DOI: 10.1073/pnas.91.12.5662 PMC Full Text View journal details
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