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PMID: 8206821 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Siderophore activity of myo-inositol hexakisphosphate in Pseudomonas aeruginosa.

Journal of bacteriology ·Vol. 176 ·No. 12 ·1994-06-00 ·Pages 3455-9

Smith AW, Poyner DR, Hughes HK, Lambert PA

Abstract

myo-Inositol hexakisphosphate (InsP6), which is found in soil and most, if not all, plant and animal cells, has been estimated to have an affinity for Fe3+ in the range of 10(25) to 10(30) M-1. In this report, we demonstrate that the Fe-InsP6 complex has siderophore activity and is able to reverse the iron-restricted growth inhibition of Pseudomonas aeruginosa by ethylene diamine di(o-hydroxyphenyl)acetic acid. With 55Fe-InsP6 in transport studies, iron uptake is strongly iron regulated, being repressed after growth in iron-replete conditions and inhibited by treatment with potassium cyanide and carbonyl cyanide m-chlorophenylhydrazone. The kinetics of iron transport revealed a Km of 100 nM. Self-displacement of binding of [3H]InsP6 to isolated membranes by InsP6 revealed a single class of binding sites (Kd = 143 +/- 6 nM; Hill coefficient, 1.1 +/- 0.1). The binding of [3H]InsP6 to membranes was not dependent on whether cells had been grown under conditions of high or low iron concentrations. We believe that this is the first report of inositol polyphosphate activity in prokaryotic cells.

MeSH Terms
Binding, Competitive Cell Division/drug effects Cell Membrane/metabolism Dose-Response Relationship, Drug Ferric Compounds/metabolism,pharmacology Iron/metabolism Phytic Acid/metabolism,pharmacology Pseudomonas aeruginosa/growth & development,metabolism Siderophores/metabolism Time Factors
Chemicals
Ferric Compounds Siderophores monoferric phytate Phytic Acid Iron
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Smith A W
Department of Pharmaceutical and Biological Sciences, Aston University, United Kingdom.
Poyner D R
Hughes H K
Lambert P A
References (30)
30 references, click to expand
  1. Mechanistically novel iron(III) transport system in Serratia marcescens.
    J Bacteriol. 1989 Jan;171(1):238-43 PMID: 2644190
  2. Iron and virulence in the family Enterobacteriaceae.
    Crit Rev Microbiol. 1988;16(2):81-111 PMID: 3067977
  3. Inositol phosphates and cell signalling.
    Nature. 1989 Sep 21;341(6239):197-205 PMID: 2550825
  4. Utilization of transferrin-bound iron by Haemophilus species of human and porcine origins.
    FEMS Microbiol Lett. 1989 Nov;53(1-2):123-7 PMID: 2533128
  5. Nucleotide sequences of the sfuA, sfuB, and sfuC genes of Serratia marcescens suggest a periplasmic-binding-protein-dependent iron transport mechanism.
    J Bacteriol. 1990 Feb;172(2):572-8 PMID: 2404942
  6. Identification of a novel inositol phosphate recognition site: specific [3H]inositol hexakisphosphate binding to brain regions and cerebellar membranes.
    Biochem Biophys Res Commun. 1990 Mar 16;167(2):819-27 PMID: 2322254
  7. Specific binding sites for inositolhexakisphosphate in brain and anterior pituitary.
    Mol Pharmacol. 1990 May;37(5):689-93 PMID: 2338945
  8. Novel two-component transmembrane transcription control: regulation of iron dicitrate transport in Escherichia coli K-12.
    J Bacteriol. 1990 Dec;172(12):6749-58 PMID: 2254251
  9. Enterobactin-mediated iron transport in Pseudomonas aeruginosa.
    J Bacteriol. 1990 Dec;172(12):6991-6 PMID: 2174865
  10. myo-inositol pentakisphosphates. Structure, biological occurrence and phosphorylation to myo-inositol hexakisphosphate.
    Biochem J. 1991 Apr 15;275 ( Pt 2):485-99 PMID: 1850990
  11. Transport of iron across the outer membrane.
    Biol Met. 1991;4(1):14-22 PMID: 1854585
  12. Iron and bacterial virulence--a brief overview.
    Biol Met. 1991;4(1):7-13 PMID: 1830211
  13. Iron transport systems of Serratia marcescens.
    J Bacteriol. 1992 Feb;174(4):1378-87 PMID: 1531225
  14. Inositol hexakisphosphate receptor identified as the clathrin assembly protein AP-2.
    Biochem Biophys Res Commun. 1992 Aug 31;187(1):158-63 PMID: 1325787
  15. Polyphosphate-selective porin OprO of Pseudomonas aeruginosa: expression, purification and sequence.
    Mol Microbiol. 1992 Aug;6(16):2319-26 PMID: 1406271
  16. Inositol polyphosphate receptor and clathrin assembly protein AP-2 are related proteins that form potassium-selective ion channels in planar lipid bilayers.
    Proc Natl Acad Sci U S A. 1992 Oct 1;89(19):8976-80 PMID: 1329085
  17. Characterization of metal ion-induced [3H]inositol hexakisphosphate binding to rat cerebellar membranes.
    J Biol Chem. 1993 Jan 15;268(2):1032-8 PMID: 8419311
  18. Isolation and characterization of two immunochemically distinct alkaline phosphatases from Pseudomonas aeruginosa.
    FEMS Microbiol Lett. 1993 Feb 1;106(3):281-6 PMID: 8454193
  19. Identification and characterization of the pupB gene encoding an inducible ferric-pseudobactin receptor of Pseudomonas putida WCS358.
    Mol Microbiol. 1993 May;8(3):591-601 PMID: 8392140
  20. Inhibition of iron-catalysed hydroxyl radical formation by inositol polyphosphates: a possible physiological function for myo-inositol hexakisphosphate.
    Biochem J. 1993 Sep 15;294 ( Pt 3):929-34 PMID: 8379947
  21. Active transport of ferric schizokinen in Anabaena sp.
    J Bacteriol. 1982 Jul;151(1):288-94 PMID: 6806241
  22. Iron withholding: a defense against infection and neoplasia.
    Physiol Rev. 1984 Jan;64(1):65-102 PMID: 6420813
  23. Evidence for a common siderophore transport system but different siderophore receptors in Neurospora crassa.
    J Bacteriol. 1985 May;162(2):715-21 PMID: 2985545
  24. Effects of siderophores on the growth of Pseudomonas aeruginosa in human serum and transferrin.
    Infect Immun. 1985 Jul;49(1):132-40 PMID: 3159677
  25. Myo-inositol transport in bacteria: H+ symport and periplasmic binding protein dependence.
    Ann N Y Acad Sci. 1985;456:351-60 PMID: 3004297
  26. Identification of inositol hexaphosphate in 31P-NMR spectra of Dictyostelium discoideum amoebae. Relevance to intracellular pH determination.
    Biochim Biophys Acta. 1987 Oct 22;931(1):16-25 PMID: 2820508
  27. Study of the siderophores and receptors in different clinical isolates of Pseudomonas aeruginosa.
    Antibiot Chemother (1971). 1987;39:290-306 PMID: 2823691
  28. Molecular mechanism of regulation of siderophore-mediated iron assimilation.
    Microbiol Rev. 1987 Dec;51(4):509-18 PMID: 2963952
  29. Identification and characterization of the human lactoferrin-binding protein from Neisseria meningitidis.
    Infect Immun. 1988 May;56(5):1144-9 PMID: 3128478
  30. Identification and characterization of the transferrin receptor from Neisseria meningitidis.
    Mol Microbiol. 1988 Mar;2(2):281-8 PMID: 3132585
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.12.3455-3459.1994
Published
1994-06-00
Pages
3455-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205531
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.176.12.3455-3459.1994 PMC Full Text View journal details
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