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PMID: 820713 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Rapid micromethod of screening for antibodies to disease agents using the indirect enzyme-labeled antibody test.

Journal of clinical microbiology ·Vol. 3 ·No. 6 ·1976-06-00 ·Pages 604-8

Saunders GC, Clinard EH

Abstract

We have developed a rapid and simple indirect enzyme-labeled antibody procedure suitable for screening for antibodies to viral, bacterial, and parasitic antigens. Disposable microplates were utilized as antigen carriers and test vehicles. The test worked equally well with serum or whole unclotted blood. Using an automatic pipettor, 96 tests could be performed in 30 to 60 min. We report on the development of enzyme-labeled antibody procedures for the detection and surveillance of animal diseases.

MeSH Terms
Animals Antibodies/analysis Antibodies, Bacterial/analysis Antibodies, Viral/analysis Brucella abortus/immunology Classical Swine Fever/microbiology Diagnosis, Differential Evaluation Studies as Topic Horseradish Peroxidase/immunology RNA Viruses/immunology Serologic Tests/methods Swine Swine Diseases/diagnosis Trichinella/immunology
Chemicals
Antibodies Antibodies, Bacterial Antibodies, Viral Horseradish Peroxidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Saunders G C
Clinard E H
References (9)
9 references, click to expand
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    J Immunol Methods. 1976;10(1):67-83 PMID: 1254975
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    FEBS Lett. 1971 Jun 24;15(3):232-236 PMID: 11945853
  9. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.
    J Immunol. 1972 Jul;109(1):129-35 PMID: 4113792
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.3.6.604-608.1976
Published
1976-06-00
Pages
604-8
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC274363
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.3.6.604-608.1976 PMC Full Text View journal details
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