Home LiteratureArticle Details
PMID: 8208614 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Murine protein which binds preferentially to oligo-C-rich single-stranded nucleic acids.

Nucleic acids research ·Vol. 22 ·No. 10 ·1994-05-25 ·Pages 1885-9

Goller M, Funke B, Gehe-Becker C, Kröger B, Lottspeich F, Horak I

Abstract

Two single-stranded nucleic acid binding proteins mCBP and mCTBP were identified by means of their binding to a potential recombination hotspot in LTRs of mouse retro-transposons. Both are nuclear proteins of 35 and 55 kDa respectively. mCBP binds preferentially to oligo dC, mCTBP to oligo dCdT. mCBP was purified and its cDNA was isolated and sequenced.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Binding Sites Cloning, Molecular DNA Transposable Elements/genetics DNA, Complementary/biosynthesis DNA, Single-Stranded/genetics,metabolism DNA-Binding Proteins/chemistry,genetics,isolation & purification,metabolism Liver/chemistry Male Mice Molecular Sequence Data Molecular Weight Mutation/genetics Nuclear Proteins/chemistry,genetics,isolation & purification,metabolism Poly C/metabolism Protein Binding Repetitive Sequences, Nucleic Acid/genetics Sequence Analysis Sequence Homology, Amino Acid Testis/chemistry
Chemicals
DNA Transposable Elements DNA, Complementary DNA, Single-Stranded DNA-Binding Proteins Nuclear Proteins mCBP protein, mouse Poly C
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Goller M
Institut für Virologie und Immunbiologie, Universität Würzburg, Germany.
Funke B
Gehe-Becker C
Kröger B
Lottspeich F
Horak I
References (15)
15 references, click to expand
  1. Characterization and primary structure of the poly(C)-binding heterogeneous nuclear ribonucleoprotein complex K protein.
    Mol Cell Biol. 1992 Jan;12(1):164-71 PMID: 1729596
  2. Paranemic structures of DNA and their role in DNA unwinding.
    Crit Rev Biochem Mol Biol. 1991;26(5-6):475-559 PMID: 1662125
  3. The pre-mRNA binding K protein contains a novel evolutionarily conserved motif.
    Nucleic Acids Res. 1993 Mar 11;21(5):1193-8 PMID: 8464704
  4. Specific binding of heterogeneous ribonucleoprotein particle protein K to the human c-myc promoter, in vitro.
    J Biol Chem. 1993 Aug 25;268(24):18249-58 PMID: 8349701
  5. hnRNP proteins and the biogenesis of mRNA.
    Annu Rev Biochem. 1993;62:289-321 PMID: 8352591
  6. Transcription-dependent recombination induced by triple-helix formation.
    Genes Dev. 1993 Sep;7(9):1766-78 PMID: 8370525
  7. The structure of DNA.
    Cold Spring Harb Symp Quant Biol. 1953;18:123-31 PMID: 13168976
  8. Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.
    Nucleic Acids Res. 1981 Dec 11;9(23):6505-25 PMID: 6275366
  9. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  10. Family of middle repetitive DNA sequences in the mouse genome with structural features of solitary retroviral long terminal repeats.
    Proc Natl Acad Sci U S A. 1983 Jun;80(11):3327-30 PMID: 6304707
  11. Affinity purification of sequence-specific DNA binding proteins.
    Proc Natl Acad Sci U S A. 1986 Aug;83(16):5889-93 PMID: 3461465
  12. Single-stranded DNA binding proteins required for DNA replication.
    Annu Rev Biochem. 1986;55:103-36 PMID: 3527040
  13. A recombination hotspot in the LTR of a mouse retrotransposon identified in an in vitro system.
    Cell. 1989 Jun 16;57(6):937-46 PMID: 2544295
  14. Altered gene expression correlates with DNA structure.
    Genes Dev. 1991 Dec;5(12B):2547-54 PMID: 1752443
  15. Formation of novel hairpin structures by telomeric C-strand oligonucleotides.
    Nucleic Acids Res. 1992 Feb 11;20(3):507-11 PMID: 1741285
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/22.10.1885
Published
1994-05-25
Pages
1885-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC308089
Subset
IM
Databases
GENBANK
X75947
External Links
PubMed source DOI: 10.1093/nar/22.10.1885 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2