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PMID: 821395 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Rickettsial cell water and membrane permeability determined by a micro space technique.

Applied and environmental microbiology ·Vol. 31 ·No. 1 ·1976-01-00 ·Pages 146-9

Winkler HH

Abstract

A micro space technique for determining membrane permeability in Rickettsia prowazeki is described and justified. The cell water, cell wall plus periplasmic volume, and glutamate, ethylene glycol, and adenosine diphosphate permeabilities were determined by this method. The effect of nonionic detergents on rickettsial permeability was examined: Triton X-100 destroyed the permeability barrier, whereas Lubrol-WX left it intact.

MeSH Terms

Adenosine Diphosphate/metabolism Bacteriological Techniques Cell Membrane Permeability/drug effects Ethylene Glycols/metabolism Glutamates/metabolism Inulin/metabolism Polyethylene Glycols/pharmacology Rickettsia prowazekii/metabolism Sucrose/metabolism Surface-Active Agents/pharmacology Water/metabolism

Chemicals

Ethylene Glycols Glutamates Surface-Active Agents Water Polyethylene Glycols Sucrose Adenosine Diphosphate Inulin

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Winkler H H

References (17)

17 references, click to expand

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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.31.1.146-149.1976

Published

1976-01-00

Pages

146-9

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC169733

Subset

External Links

PubMed source DOI: 10.1128/aem.31.1.146-149.1976 PMC Full Text View journal details

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Rickettsial cell water and membrane permeability determined by a micro space technique.

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