Home LiteratureArticle Details
PMID: 8216232 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Involvement of the proteasome and antizyme in ornithine decarboxylase degradation by a reticulocyte lysate.

The Biochemical journal ·Vol. 295 ( Pt 1) ·1993-10-01 ·Pages 305-8

Murakami Y, Matsufuji S, Tanaka K, Ichihara A, Hayashi S

Abstract

Ornithine decarboxylase (ODC) degradation in a freshly prepared reticulocyte lysate was examined. Immunodepletion of proteasomes from the reticulocyte lysate resulted in almost complete loss of ODC degradation. In contrast with the previously reported degradation in extracts of hepatoma tissue-culture (HTC) and Chinese-hamster ovary (CHO) cells or that by the purified 26 S proteasome, efficient degradation of ODC was observed in the lysate without exogenous antizyme, an ODC protein inhibitor induced by polyamines, owing to the presence of a significant amount of antizyme in the lysate. The degradation of ODC in the lysate was strongly suppressed on inactivation of antizyme in the lysate with antizyme inhibitor, a protein which binds to the antizyme and releases ODC from the ODC-antizyme complex. Thus the main pathway for ODC degradation in a reticulocyte lysate was essentially the same as that characterized previously in extracts of HTC and CHO cells, namely an ATP- and antizyme-dependent 26 S proteasome-catalysed pathway that is presumed to be responsible for ODC degradation in whole cells.

MeSH Terms
Animals Carrier Proteins Cell-Free System Cysteine Endopeptidases/metabolism Mice Multienzyme Complexes/metabolism Ornithine Decarboxylase/metabolism Ornithine Decarboxylase Inhibitors Proteasome Endopeptidase Complex Proteins/metabolism,pharmacology Rabbits Reticulocytes/metabolism
Chemicals
Carrier Proteins Multienzyme Complexes Ornithine Decarboxylase Inhibitors Proteins ornithine decarboxylase antizyme ornithine decarboxylase antizyme inhibitor Cysteine Endopeptidases Proteasome Endopeptidase Complex Ornithine Decarboxylase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Murakami Y
Department of Nutrition, Jikei University School of Medicine, Tokyo, Japan.
Matsufuji S
Tanaka K
Ichihara A
Hayashi S
References (30)
30 references, click to expand
  1. The appearance of an ornithine decarboxylase inhibitory protein upon the addition of putrescine to cell cultures.
    Biochim Biophys Acta. 1976 Apr 23;428(2):456-65 PMID: 179575
  2. Multiple regulation of ornithine decarboxylase in enzyme-overproducing cells.
    Biochem J. 1993 Jan 15;289 ( Pt 2):581-6 PMID: 8424799
  3. A macromolecular inhibitor of the antizyme to ornithine decarboxylase.
    Biochem J. 1982 Jun 15;204(3):647-52 PMID: 7126159
  4. Components of ubiquitin-protein ligase system. Resolution, affinity purification, and role in protein breakdown.
    J Biol Chem. 1983 Jul 10;258(13):8206-14 PMID: 6305978
  5. Role of antizyme in degradation of ornithine decarboxylase in HTC cells.
    Biochem J. 1985 Mar 15;226(3):893-6 PMID: 4039135
  6. Two-step purification of mouse kidney ornithine decarboxylase.
    Prep Biochem. 1988;18(2):227-38 PMID: 3131757
  7. Proteasomes (multi-protease complexes) as 20 S ring-shaped particles in a variety of eukaryotic cells.
    J Biol Chem. 1988 Nov 5;263(31):16209-17 PMID: 3141402
  8. Purification and characterization of antizyme inhibitor of ornithine decarboxylase from rat liver.
    Biochim Biophys Acta. 1989 Apr 25;991(1):44-9 PMID: 2713421
  9. Properties and fluctuations in vivo of rat liver antizyme inhibitor.
    Biochem J. 1989 May 1;259(3):839-45 PMID: 2730588
  10. Degradation of ornithine decarboxylase in reticulocyte lysate is ATP-dependent but ubiquitin-independent.
    J Biol Chem. 1989 Sep 25;264(27):15949-52 PMID: 2550429
  11. Polyamine-mediated regulation of mouse ornithine decarboxylase is posttranslational.
    Mol Cell Biol. 1989 Dec;9(12):5484-90 PMID: 2511435
  12. Influence of the 5'-untranslated region of ornithine decarboxylase mRNA and spermidine on ornithine decarboxylase synthesis.
    J Biol Chem. 1990 Aug 5;265(22):13036-41 PMID: 2376585
  13. The E6 oncoprotein encoded by human papillomavirus types 16 and 18 promotes the degradation of p53.
    Cell. 1990 Dec 21;63(6):1129-36 PMID: 2175676
  14. Degradation of nuclear oncoproteins by the ubiquitin system in vitro.
    Proc Natl Acad Sci U S A. 1991 Jan 1;88(1):139-43 PMID: 1846034
  15. Analyses of ornithine decarboxylase antizyme mRNA with a cDNA cloned from rat liver.
    J Biochem. 1990 Sep;108(3):365-71 PMID: 2148936
  16. Characterization of sequences involved in mediating degradation of ornithine decarboxylase in cells and in reticulocyte lysate.
    Eur J Biochem. 1991 Mar 28;196(3):647-51 PMID: 2013288
  17. The C terminus of mouse ornithine decarboxylase confers rapid degradation on dihydrofolate reductase. Support for the pest hypothesis.
    J Biol Chem. 1991 Jun 15;266(17):11213-20 PMID: 2040628
  18. Identification of residues in ornithine decarboxylase essential for enzymic activity and for rapid protein turnover.
    Biochem J. 1991 Aug 1;277 ( Pt 3):671-5 PMID: 1872802
  19. cis-recognition and degradation of ornithine decarboxylase subunits in reticulocyte lysate.
    Biochem J. 1991 Aug 1;277 ( Pt 3):683-5 PMID: 1872804
  20. Degradation of p53 can be targeted by HPV E6 sequences distinct from those required for p53 binding and trans-activation.
    Cell. 1991 Nov 1;67(3):547-56 PMID: 1657399
  21. A cellular protein mediates association of p53 with the E6 oncoprotein of human papillomavirus types 16 or 18.
    EMBO J. 1991 Dec;10(13):4129-35 PMID: 1661671
  22. Structural elements of ornithine decarboxylase required for intracellular degradation and polyamine-dependent regulation.
    Mol Cell Biol. 1992 May;12(5):2178-85 PMID: 1569947
  23. Antizyme, a protein induced by polyamines, accelerates the degradation of ornithine decarboxylase in Chinese-hamster ovary-cell extracts.
    Biochem J. 1992 May 1;283 ( Pt 3):661-4 PMID: 1590755
  24. Demonstration that a human 26S proteolytic complex consists of a proteasome and multiple associated protein components and hydrolyzes ATP and ubiquitin-ligated proteins by closely linked mechanisms.
    Eur J Biochem. 1992 Jun 1;206(2):567-78 PMID: 1317798
  25. Mouse ornithine decarboxylase is stable in Trypanosoma brucei.
    J Biol Chem. 1992 Jun 5;267(16):11034-7 PMID: 1597444
  26. Destabilization of ornithine decarboxylase by transfected antizyme gene expression in hepatoma tissue culture cells.
    J Biol Chem. 1992 Jul 5;267(19):13138-41 PMID: 1618815
  27. Interaction of the human papillomavirus type 16 E6 oncoprotein with wild-type and mutant human p53 proteins.
    J Virol. 1992 Aug;66(8):5100-5 PMID: 1321290
  28. Ornithine decarboxylase is degraded by the 26S proteasome without ubiquitination.
    Nature. 1992 Dec 10;360(6404):597-9 PMID: 1334232
  29. Targeted degradation of c-Fos, but not v-Fos, by a phosphorylation-dependent signal on c-Jun.
    Science. 1992 Dec 18;258(5090):1941-4 PMID: 1470918
  30. An efficient mRNA-dependent translation system from reticulocyte lysates.
    Eur J Biochem. 1976 AUG 1;67(1):247-56 PMID: 823012
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2950305
Published
1993-10-01
Pages
305-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1134853
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2950305 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2