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PMID: 822115 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The Ly-3 antigens on mouse thymocytes: immune precipitation and molecular weight characterization.

The Journal of experimental medicine ·Vol. 144 ·No. 2 ·1976-08-01 ·Pages 476-93

Durda PJ, Gottlieb PD

Abstract

Specific anti-Ly sera were employed to precipitate Ly antigens from Nonidet P-40 extracts of mouse thymocytes labeled with 125I using lactoperoxidase and with NaB3H4 using galactose oxidase. Thymocytes from mice of the congenic strains C57BL/6J (Ly-2.2, Ly-3.2 positive), C57BL/6Ly-2a, Ly-3a (Ly-2.1, Ly-3.1 positive) and C57BL/6-Ly-2a (Ly-2.1, Ly-3.2-positive) were used as sources of labeled antigens and as immune adsorbants to permit evaluation of the specificity of each anti-Ly serum employed. Results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions are consistent with the Ly-3.1 antigen containing a glycoprotein subunit with an apparent mol wt of 35,000 daltons. Specific precipitates obtained using anti-Ly-2.1 serum yielded SDS-PAGE profiles identical to that obtained with anti-Ly-3.1 serum, suggesting that the Ly-2 and Ly-3 antigens have the same molecular weight distribution. The relationships of these results to the observed close genetic and topological linkage of Ly-2 and Ly-3 and to the genetic linkage of these loci with the IB-peptide marker, a mouse Bk-region polymorphism, are discussed.

MeSH Terms
Animals Antigen-Antibody Complex Chromatography, Gel Electrophoresis, Polyacrylamide Gel Galactose/analysis Genetic Linkage Glycoproteins/analysis Immunoglobulin kappa-Chains/biosynthesis Isoantigens/analysis Mice Mice, Inbred C57BL Molecular Weight Pronase Surface Properties T-Lymphocytes/immunology Thymus Gland/immunology Trypsin
Chemicals
Antigen-Antibody Complex Glycoproteins Immunoglobulin kappa-Chains Isoantigens Trypsin Pronase Galactose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Durda P J
Gottlieb P D
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30 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.144.2.476
Published
1976-08-01
Pages
476-93
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2190382
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.144.2.476 PMC Full Text View journal details
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