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PMID: 8248240 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A ubiquitous protein is the source of naturally occurring peptides that are recognized by a CD8+ T-cell clone.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 23 ·1993-12-01 ·Pages 11272-6

Udaka K, Tsomides TJ, Walden P, Fukusen N, Eisen HN

Abstract

We previously isolated from mouse spleen an octapeptide (LSPFPFDL) that in association with the class I major histocompatibility complex protein Ld is recognized by the antigen-specific receptor of an alloreactive CD8+ T-cell clone (2C). Guided by an assay dependent upon the same 2C T-cell receptor, we have now isolated from the same source another naturally occurring peptide. The second peptide (VAITRIEQLSPFPFDL) includes the entire octapeptide sequence and preliminary evidence suggests that it may be a natural precursor of the octapeptide. On finding extensive sequence homology between the 16-mer and part of human 2-oxoglutarate dehydrogenase, we determined the cDNA sequence of mouse 2-oxoglutarate dehydrogenase and found that the deduced amino acid sequence matches precisely the two naturally occurring peptides, indicating their origin by cellular processing of this ubiquitous self protein.

MeSH Terms
Amino Acid Sequence Animals Base Sequence CD8 Antigens/analysis Clone Cells Cytotoxicity, Immunologic DNA Primers/chemistry Fungal Proteins/chemistry,immunology Humans Ketoglutarate Dehydrogenase Complex/chemistry,immunology Mice Mice, Inbred BALB C Molecular Sequence Data Peptides/chemistry,immunology Protein Precursors/chemistry,immunology Swine T-Lymphocyte Subsets/cytology,immunology
Chemicals
CD8 Antigens DNA Primers Fungal Proteins Peptides Protein Precursors Ketoglutarate Dehydrogenase Complex
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Udaka K
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Tsomides T J
Walden P
Fukusen N
Eisen H N
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.23.11272
Published
1993-12-01
Pages
11272-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC47964
Subset
IM
Grants
NCI NIH HHS · CA14051 · United States
NCI NIH HHS · R35-CA42504 · United States
NCRR NIH HHS · RR00317 · United States
Databases
GENBANK
U02971
External Links
PubMed source DOI: 10.1073/pnas.90.23.11272 PMC Full Text View journal details
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