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PMID: 8284208 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Monovalent cation effects on intermolecular purine-purine-pyrimidine triple-helix formation.

Nucleic acids research ·Vol. 21 ·No. 24 ·1993-12-11 ·Pages 5630-5

Cheng AJ, Van Dyke MW

Abstract

The binding of a 19-mer guanosine-rich oligodeoxyribonucleotide, TG3TG4TG4TG3T (ODN 1), to a complementary polypurine DNA target was investigated by DNase I footprinting and restriction endonuclease protection assays. Monovalent cations inhibited intermolecular purine-purine-pyrimidine triple-helical DNA formation, with K+ and Rb+ being most effective, followed by NH4+ and Na+. Li+ and Cs+ had little to no effect. Similar results were observed with the G/A-rich oligonucleotide AG3AG4AG4AG3AGCT. Kinetic studies indicated that monovalent cations interfered with oligonucleotide-duplex DNA association but did not significantly promote triplex dissociation. The observed order of monovalent cation inhibition of triplex formation is reminiscent of their effect on tetraplex formation with G/T-rich oligonucleotides. However, using electrophoretic mobility shift assays we found that the oligonucleotide ODN 1 did not appear to form a four-stranded species under conditions promoting tetraplex formation. Taken together, our data suggest that processes other than the self-association of oligonucleotides into tetraplexes might be involved in the inhibitory effect of monovalent cations on purine-pyrimidine-purine triplex formation.

MeSH Terms
Base Sequence Cations Kinetics Magnesium/chemistry Molecular Sequence Data Nucleic Acid Conformation Oligodeoxyribonucleotides/chemistry Potassium/chemistry Purines/chemistry Pyrimidines/chemistry
Chemicals
Cations Oligodeoxyribonucleotides Purines Pyrimidines Magnesium Potassium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cheng A J
Department of Tumor Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Van Dyke M W
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.24.5630
Published
1993-12-11
Pages
5630-5
Language
English
Region
England
NLM ID
0411011
PMCID
PMC310527
Subset
IM
Grants
NCI NIH HHS · CA16672 · United States
External Links
PubMed source DOI: 10.1093/nar/21.24.5630 PMC Full Text View journal details
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