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PMID: 3671067 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Adenine specific DNA chemical sequencing reaction.

Nucleic acids research ·Vol. 15 ·No. 19 ·1987-10-12 ·Pages 7823-30

Iverson BL, Dervan PB

Abstract

Reaction of DNA with K2PdCl4 at pH 2.0 followed by a piperidine workup produces specific cleavage at adenine (A) residues. Product analysis revealed the K2PdCl4 reaction involves selective depurination at adenine, affording an excision reaction analogous to the other chemical DNA sequencing reactions. Adenine residues methylated at the exocyclic amine (N6) react with lower efficiency than unmethylated adenine in an identical sequence. This simple protocol specific for A may be a useful addition to current chemical sequencing reactions.

MeSH Terms
Adenine Base Sequence Chromatography, High Pressure Liquid DNA/analysis DNA, Bacterial/analysis Palladium Piperidines Plasmids
Chemicals
DNA, Bacterial Piperidines tetrachloropalladium Palladium piperidine DNA Adenine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Iverson B L
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena 91125.
Dervan P B
References (6)
6 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.19.7823
Published
1987-10-12
Pages
7823-30
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306310
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.19.7823 PMC Full Text View journal details
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