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PMID: 8306821 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The RNA polymerase I-specific transcription initiation factor UBF is associated with transcriptionally active and inactive ribosomal genes.

Chromosoma ·Vol. 102 ·No. 9 ·1993-11-00 ·Pages 599-611

Zatsepina OV, Voit R, Grummt I, Spring H, Semenov MV, Trendelenburg MF

Abstract

We have characterized an anti-NOR (nucleolar organizer region) serum (P419) from a patient with rheumatoid arthritis and show that it contains antibodies directed against the RNA polymerase I-specific transcription initiation factor UBF. This serum reacts with UBF from a variety of vertebrate cells as revealed both by immunoblotting and by indirect immunofluorescence. We have used the P419 serum to study the intracellular localization of this transcription factor at the light and electron microscopic level. In interphase cells, UBF exhibits a pronounced punctate pattern and is found to be associated with necklace-like structures, which appear to reflect the transcriptionally active state of the nucleolus. Inhibition of rRNA synthetic activity caused either by nutritional starvation or by actinomycin D treatment resulted in a marked decrease in the number and in a significant increase in the size of UBF-positive granules. Under all experimental conditions applied, UBF was exclusively found within the nucleolus and was not released into the nucleoplasm or cytoplasm. During mitosis, UBF was found to be concentrated at the chromosomal NOR indicating that a significant quantity, if not all, of this factor remains bound to the ribosomal transcription units. From this we conclude that UBF is associated both with transcriptionally active and inactive rRNA genes and, therefore, changes in the intracellular localization of UBF are very likely not involved in rDNA transcription regulation.

MeSH Terms
Animals Arthritis, Rheumatoid/immunology Autoantibodies Cattle Cell Line Cell Nucleolus/metabolism Cricetinae DNA, Ribosomal/genetics,metabolism DNA-Binding Proteins/immunology,metabolism Dactinomycin/pharmacology Dichlororibofuranosylbenzimidazole/pharmacology Dipodomys Fluorescent Antibody Technique Humans Interphase Mice Microscopy, Immunoelectron Mitosis Nucleolus Organizer Region/immunology,metabolism Pol1 Transcription Initiation Complex Proteins RNA Polymerase I/metabolism Rats Swine Transcription Factors/immunology,metabolism Transcription, Genetic Xenopus laevis
Chemicals
Autoantibodies DNA, Ribosomal DNA-Binding Proteins Pol1 Transcription Initiation Complex Proteins Transcription Factors transcription factor UBF Dactinomycin Dichlororibofuranosylbenzimidazole RNA Polymerase I
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Zatsepina O V
A. N. Belozersky Institute of Physical and Chemical Biology, Moscow State University, Russia.
Voit R
Grummt I
Spring H
Semenov M V
Trendelenburg M F
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00352307
Published
1993-11-00
Pages
599-611
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00352307 View journal details
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